Meyers R L, Pettit T H
J Immunol. 1975 Apr;114(4):1269-74.
Experimental allergic unveitis (EAU) was produced in strain 13 guinea pigs after immunization with purified guinea pig retinal rod outer segments and pigment epithelium (PE) in mycobacterial adjuvant emulsion. The lesions of EAU appear as inflammatory infiltrates of the iris, ciliary body, and choroid, often with photorecptor degeneration. Specific antibodies are frequently detected in the serum of some but not of all animals with clinical uveitis. Immunohistochemical staining of normal and inflamed eyes with serum from the immunized animals with clinical disease demonstrated specific antigens localized in the retinal photoreceptor layers, whether or not circulating precipitating antibodies were also present in the serum. All immunized animals demonstrated delayed-type hypersensitivity (DTH) characterized by skin reactions of mononuclear cells and by the vitro inhibition of migration of cells from the sensitized animals in the presence of specific antigen whether or not clinical uveitis occurred. However, stronger DTH reactions were observed during clinical uveitis. Cellular immunity appears to correlate with clinical EAU, whereas, no correlation was found with serum antibody. These findings suggest that the retinal photoreceptor cell and PE are the source of the significant antigens in autoimmune uveitis and retinitis.
用纯化的豚鼠视网膜视杆细胞外节和色素上皮（PE）在分枝杆菌佐剂乳剂中免疫13系豚鼠后，诱发实验性变应性葡萄膜炎（EAU）。EAU的病变表现为虹膜、睫状体和脉络膜的炎性浸润，常伴有光感受器变性。在部分但并非所有患有临床葡萄膜炎的动物血清中经常检测到特异性抗体。用患有临床疾病的免疫动物血清对正常和发炎的眼睛进行免疫组织化学染色显示，无论血清中是否也存在循环沉淀抗体，特异性抗原都定位于视网膜光感受器层。所有免疫动物均表现出迟发型超敏反应（DTH），其特征为单核细胞的皮肤反应以及在存在特异性抗原的情况下，致敏动物细胞体外迁移受到抑制，无论是否发生临床葡萄膜炎。然而，在临床葡萄膜炎期间观察到更强的DTH反应。细胞免疫似乎与临床EAU相关，而与血清抗体无关。这些发现表明，视网膜光感受器细胞和PE是自身免疫性葡萄膜炎和视网膜炎中重要抗原的来源。
