Freyssinet J M, Gauchy J, Spaethe R, Cazenave J P
Haemostasis. 1987;17(3):114-20. doi: 10.1159/000215568.
Substantial thrombomodulin activity could be detected in tissue thromboplastin preparations from placenta or from lung but not from brain. When the amount of these preparations was adjusted to contain 1 unit of tissue factor activity, up to 0.85 units of thrombomodulin activity could be measured, corresponding to the generation of 17 pmol/ml/min of activated protein C when 1.5 microM human protein C was activated by 20 nM human alpha-thrombin in the presence of 5 mM CaCl2. After treatment by phospholipase C, thrombomodulin activity was reduced in these samples. Addition of mixed brain procoagulant phospholipids partially restored thrombomodulin activity in the phospholipase C-treated samples. These results emphasize the role of phospholipids in the expression of optimal thrombomodulin activity in tissue thromboplastin preparations from placenta or from lung.
在来自胎盘或肺的组织凝血活酶制剂中可检测到大量的血栓调节蛋白活性，但在来自脑的制剂中则检测不到。当将这些制剂的量调整为含有1个单位的组织因子活性时，可测得高达0.85个单位的血栓调节蛋白活性，这相当于在5 mM氯化钙存在的情况下，当1.5 microM人蛋白C被20 nM人α-凝血酶激活时，每分钟产生17 pmol/ml的活化蛋白C。经磷脂酶C处理后，这些样品中的血栓调节蛋白活性降低。添加混合的脑促凝磷脂可部分恢复经磷脂酶C处理的样品中的血栓调节蛋白活性。这些结果强调了磷脂在胎盘或肺的组织凝血活酶制剂中最佳血栓调节蛋白活性表达中的作用。
