欧洲赤松种子萌发过程中环磷酸腺苷及相关酶的出现情况。
Occurrence of cyclic AMP and related enzymes during germination of Pinus pinea seeds.
作者信息
Martelli P, Lusini P, Bovalini L, Bartali R, Franchi G G, Cinci G
出版信息
Ital J Biochem. 1987 May-Jun;36(3):188-93.
The occurrence of cAMP, adenylate cyclase and cAMP phosphodiesterase has been tested in Pinus pinea seed during germination. The study has been carried out on dormant and imbibed seeds, seedlings, endospermic residues, roots and cotyledons. cAMP has been detected by the protein binding method and its occurrence has been verified by HPLC detections. cAMP phosphodiesterase shows a very high activity at acidic pH, while being completely inactive at pH 7.4. At this pH value, well detectable levels of adenylate cyclase have been observed. Therefore, the classical pathway of synthesis and breakdown of cAMP, already accepted for animal and bacterial cells, seems to be operating in Pinus pinea plant too.
在欧洲赤松种子萌发过程中，对环磷酸腺苷（cAMP）、腺苷酸环化酶和cAMP磷酸二酯酶的存在情况进行了检测。研究对象包括休眠种子和吸胀种子、幼苗、胚乳残渣、根和子叶。采用蛋白质结合法检测cAMP，并通过高效液相色谱检测对其存在情况进行了验证。cAMP磷酸二酯酶在酸性pH条件下表现出非常高的活性，而在pH 7.4时则完全无活性。在这个pH值下，已观察到腺苷酸环化酶有可检测到的水平。因此，动物和细菌细胞中已被认可的cAMP合成与分解的经典途径，似乎在欧洲赤松植物中也在发挥作用。
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