Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University; Key Laborotory of Exploration and Utilization of Aquatic Genetic Resources; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Institute of Evolution & Marine Biodiversity, Key Laboratory of Mariculture of the Education Ministry of China, Ocean University of China, Qingdao 266003, China.
Institute of Evolution & Marine Biodiversity, Key Laboratory of Mariculture of the Education Ministry of China, Ocean University of China, Qingdao 266003, China.
Eur J Protistol. 2019 Feb;67:1-14. doi: 10.1016/j.ejop.2018.10.001. Epub 2018 Oct 13.
The living morphology, infraciliature and silverline system of two small Euplotes species, E. wuhanensis sp. n. and E. muscicola Kahl, 1932, isolated from Wuhan, central China, were investigated. Euplotes wuhanensis sp. n. is characterized by a combination of features including small size (40-50 × 25-30 μm), two conspicuously small and eight normal-sized frontoventral cirri, five transverse cirri in two groups, two marginal and two caudal cirri, seven dorsal kineties with about 12 dikinetids in the mid-dorsal row and a double-eurystomus type of dorsal silverline pattern. The Wuhan population of E. muscicola closely resembles previously described populations. The establishments of three subspecies of E. muscicola are not supported. The small subunit ribosomal RNA gene sequences were determined for both species. We propose that the two sequences under the name of E. muscicola (No. AJ305254, DQ917684 deposited in GenBank) are very likely from misidentified material. Phylogenetic analyses based on these data support the validity of both E. muscicola and E. wuhanensis as distinct species.
本研究调查了采自中国中部武汉市的 2 种小型真游仆虫(Euplotes wuhanensis sp. n. 和 E. muscicola Kahl,1932)的活体形态、纤毛图式和银线系统。E. wuhanensis sp. n. 的特征为体型较小(40-50×25-30μm),前腹刚毛 2 对小、8 对大,横棘毛 5 对分 2 组,缘棘毛 2 对,尾棘毛 2 对,背腹棘毛 7 对,背中部有 12 对双联体,具双膨型背银线。武汉的 E. muscicola 种群与先前描述的种群非常相似。不支持建立 E. muscicola 的 3 个亚种。测定了这 2 个种的小亚基核糖体 RNA 基因序列。我们认为以 E. muscicola 名义(GenBank 注册号为 AJ305254 和 DQ917684)发表的序列很可能来自错误鉴定的材料。基于这些数据的系统发育分析支持 E. muscicola 和 E. wuhanensis 为有效种。