Choudhary Dhiraj Kumar, Mishra Abha
a School of Biochemical Engineering , Indian Institute of Technology (BHU) , Varanasi , India.
Prep Biochem Biotechnol. 2018;48(10):920-929. doi: 10.1080/10826068.2018.1525560. Epub 2018 Nov 2.
Imbalance of free radicals over antioxidants in human body may result in oxidative damage to biomolecules (lipids, proteins, DNA) that causes severe chronic diseases. The aim of this proposed research was to examine the hypoglycemic and oxidative stress potential of fava bean (Vicia faba L.) seed extract. Acetone extract showed a significant effect on glucose uptake rate (77.28 ± 2.42%) in yeast cells at 25 mM glucose concentration. Minimum glucose uptake rate was found to be 52.36 ± 2.06% % by chloroform seed extract. Atomic force microscopy revealed that 3% hydrogen peroxide concentration results in roughness was found to be maximum (441 ± 6.7 nm) and along with extract treatment showed a significant reduction in roughness (251 ± 6.2 nm). Propidium iodide and DAPI staining showed apoptotic ratio as 0.40 (40 ± 1.18%,) and 0.42 (42 ± 1.16%) in hydrogen peroxide treated cell only as compared to other treatments. MTT assay showed that acetone extract had maximum survival rate (82.067%) and least survival rate was found in chloroform extract (70.48%). Hypoglycaemic potential and oxidative stress might be polyphenols (phenolics, flavonoids) present in seed extract or synergistic effect.
人体内自由基与抗氧化剂失衡可能导致生物分子（脂质、蛋白质、DNA）发生氧化损伤，进而引发严重的慢性疾病。本研究的目的是检测蚕豆（Vicia faba L.）种子提取物的降血糖和氧化应激潜力。在25 mM葡萄糖浓度下，丙酮提取物对酵母细胞的葡萄糖摄取率有显著影响（77.28 ± 2.42%）。氯仿种子提取物的最低葡萄糖摄取率为52.36 ± 2.06%。原子力显微镜显示，3%过氧化氢浓度导致的粗糙度最大（441 ± 6.7 nm），而提取物处理后粗糙度显著降低（251 ± 6.2 nm）。碘化丙啶和DAPI染色显示，仅过氧化氢处理的细胞凋亡率为0.40（40 ± 1.18%），而与其他处理相比，该处理的凋亡率为0.42（42 ± 1.16%）。MTT法显示，丙酮提取物的存活率最高（82.067%），氯仿提取物的存活率最低（70.48%）。降血糖潜力和氧化应激可能是种子提取物中存在的多酚（酚类、黄酮类）或协同效应所致。
