多聚磷酸盐以剂量依赖的方式对大鼠成骨细胞 ROS17/2.8 细胞矿化产生双重影响。
Dual effect of polyphosphate on mineralization of rat osteoblast ROS17/2.8 cells in a dose-dependent manner.
机构信息
Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Chuo-ku, Niigata-shi, Niigata 951-8122, Japan.
Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai Chiyoda-ku, Tokyo 101-8310, Japan.
出版信息
J Pharmacol Sci. 2018 Nov;138(3):209-213. doi: 10.1016/j.jphs.2018.10.002. Epub 2018 Oct 13.
Inorganic polyphosphate (polyP), a linear polymer of orthophosphate, is found at high concentrations in osteoblasts. We demonstrated the effects of various polyP concentrations on the mineralization of rat osteoblast ROS17/2.8 cells. Mineralization of ROS17/2.8 was induced by a high polyP concentration (1 mg/mL), which was accompanied by an upregulation of the bone sialoprotein and osteocalcin. In contrast, a low polyP concentration (1 × 10 mg/mL) reduced mineralization without affecting the osteogenic gene expression. Furthermore, gene expression profiling and forced expression analysis indicated that phosphodiesterase 11a could be a candidate involved in the dose-dependent effect of polyP on osteoblast mineralization.
无机多聚磷酸盐（polyP）是一种正磷酸盐的线性聚合物，在成骨细胞中浓度很高。我们研究了不同多聚磷酸盐浓度对大鼠成骨细胞 ROS17/2.8 矿化的影响。高浓度多聚磷酸盐（1mg/mL）诱导 ROS17/2.8 矿化，同时骨涎蛋白和骨钙素的表达上调。相反，低浓度多聚磷酸盐（1×10mg/mL）减少矿化，而不影响成骨基因表达。此外，基因表达谱分析和强制表达分析表明，磷酸二酯酶 11a 可能是多聚磷酸盐对成骨细胞矿化的剂量依赖性作用的候选基因。
Zotero 插件