碱性磷酸酶以细胞类型无关的方式决定多聚磷酸盐诱导的矿化作用。
Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner.
作者信息
Mikami Yoshikazu, Tsuda Hiromasa, Akiyama Yuko, Honda Masaki, Shimizu Noriyoshi, Suzuki Naoto, Komiyama Kazuo
机构信息
Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
出版信息
J Bone Miner Metab. 2016 Nov;34(6):627-637. doi: 10.1007/s00774-015-0719-6. Epub 2015 Oct 16.
Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various cells. Poly(P) concentrations of 1 and 0.5 mg/mL yielded high levels of mineralization in ROS17/2.8 osteoblast cells. Similarly, Poly(P) induced mineralization in cell types expressing alkaline phosphatase (ALP), namely, ATDC5 and MC3T3-E1, but not in CHO, C3H10T1/2, C2C12, and 3T3-L1 cells. Furthermore, forced expression of ALP caused Poly(P)-induced mineralization in CHO cells. These results suggest that ALP determines Poly(P)-induced mineralization in a cell-type independent manner.
多聚磷酸盐[Poly(P)]对成骨细胞矿化具有积极作用；然而，其潜在机制仍不清楚。此外，Poly(P)是否促进软组织矿化尚不清楚。我们通过使用各种细胞对此进行了研究。1和0.5mg/mL的Poly(P)浓度在ROS17/2.8成骨细胞中产生了高水平的矿化。同样，Poly(P)在表达碱性磷酸酶(ALP)的细胞类型（即ATDC5和MC3T3-E1）中诱导矿化，但在CHO、C3H10T1/2、C2C12和3T3-L1细胞中未诱导矿化。此外，ALP的强制表达导致CHO细胞中Poly(P)诱导的矿化。这些结果表明，ALP以细胞类型独立的方式决定Poly(P)诱导的矿化。
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