Huang Ning, Pu Xiaoming, Zhang Jingxin, Shen Huifang, Yang Qiyun, Wang Zhongwen, Lin Birun
College of Agriculture, Guangxi University, 100 Daxue Road, Nanning, 530004, China.
Guangdong Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Curr Microbiol. 2019 Jan;76(1):100-107. doi: 10.1007/s00284-018-1593-y. Epub 2018 Nov 2.
Bacterial soft rot caused by Dickeya zeae MS1 (Erwinia chrysanthemi) is one of the most devastating banana diseases worldwide. However, knowledge of the development and ecological interactions of D. zeae MS1 biofilm is limited. Here, we visualized the development and architecture of D. zeae MS1 biofilm using confocal laser scanning microscopy, and we evaluated the ability of D. zeae MS1 to form biofilms under different environmental conditions (carbon sources, temperatures, pH levels and mineral elements) using a microtiter plate assay. We found that the development of D. zeae MS1 biofilm could be categorized into four phases and that mature biofilm consisted of a highly organized architecture of both bacterial cells and a self-produced matrix of extracellular polysaccharides. Furthermore, sucrose was the most suitable carbon source for supporting the growth of biofilm cells and that 32 °C and pH 7.0 were the most favorable of the temperatures and pH levels examined. Meanwhile, the addition of Ca, Fe, K and Na enhanced the formation of biofilm in minimal medium cultures, whereas 2.5 mM Cu and Mn was inhibitory. A better understanding of biofilm formation under different environmental parameters will improve our knowledge of the growth kinetics of D. zeae MS1 biofilm.
由菊欧文氏菌（Dickeya zeae MS1，原名菊苣欧文氏菌）引起的细菌性软腐病是全球最具毁灭性的香蕉病害之一。然而，关于菊欧文氏菌MS1生物膜的发育及生态相互作用的了解却很有限。在此，我们利用共聚焦激光扫描显微镜观察了菊欧文氏菌MS1生物膜的发育及结构，并使用微孔板分析法评估了菊欧文氏菌MS1在不同环境条件（碳源、温度、pH值和矿质元素）下形成生物膜的能力。我们发现菊欧文氏菌MS1生物膜的发育可分为四个阶段，成熟生物膜由细菌细胞和自行产生的细胞外多糖基质组成的高度有序结构构成。此外，蔗糖是支持生物膜细胞生长的最适宜碳源，在所检测的温度和pH值中，32℃和pH 7.0最为有利。同时，在基本培养基培养中添加钙、铁、钾和钠可增强生物膜的形成，而2.5 mM的铜和锰则具有抑制作用。更好地了解不同环境参数下生物膜的形成将增进我们对菊欧文氏菌MS1生物膜生长动力学的认识。
