改良组蛋白肽芯片在染色质研究中的应用。

Application of modified histone peptide arrays in chromatin research.

机构信息

Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, Stuttgart University, Allmandring 31, 70550, Stuttgart, Germany.

出版信息

Arch Biochem Biophys. 2019 Jan;661:31-38. doi: 10.1016/j.abb.2018.10.019. Epub 2018 Nov 2.

DOI:10.1016/j.abb.2018.10.019

PMID:30391375

Abstract

Various post-translational modifications (PTMs) have been identified on histone proteins, which occur at hundreds of different sites. Histone PTMs influence the chromatin structure and serve as binding sites for reading domains, which further mediate downstream effects. Histone PTM antibodies or recombinant proteins derived from reading domains are unique research reagents essentially required to study histone modifications. To validate their specificity, histone PTM peptide arrays are used, because they allow to investigate the binding of proteins to a large number of different peptides in one experiment. Furthermore, histone PTM peptide arrays can be used to characterize reading domains and study the specificity of histone modifying enzymes. Here, we provide an overview of histone PTM peptide arrays, highlight some of their applications and compare different commercial histone PTM peptide arrays, viz. MODified Histone Peptide Array, AbSurance Pro Histone Peptide Microarrays, EpiTriton Histone Peptide Array and Histone Code Microarrays. These arrays contain histone peptides with several post-translational modifications in many different combinations, but they differ in peptide synthesis and immobilization methods, peptide and PTM coverage, and PTM combinatorial potential. In addition, some special applications of histone PTM peptide arrays like custom arrays or double peptide arrays are described.

摘要

各种翻译后修饰（PTMs）已在组蛋白蛋白上被鉴定出来，这些修饰发生在数百个不同的位点上。组蛋白 PTM 影响染色质结构，并作为阅读结构域的结合位点，进一步介导下游效应。组蛋白 PTM 抗体或来自阅读结构域的重组蛋白是研究组蛋白修饰所必需的独特研究试剂。为了验证其特异性，使用组蛋白 PTM 肽阵列，因为它们允许在一个实验中研究蛋白质与大量不同肽的结合。此外，组蛋白 PTM 肽阵列可用于表征阅读结构域并研究组蛋白修饰酶的特异性。在这里，我们提供了组蛋白 PTM 肽阵列的概述，强调了它们的一些应用，并比较了不同的商业组蛋白 PTM 肽阵列，即 MODified Histone Peptide Array、AbSurance Pro Histone Peptide Microarrays、EpiTriton Histone Peptide Array 和 Histone Code Microarrays。这些阵列包含具有多种翻译后修饰的组蛋白肽，这些修饰在许多不同的组合中，但它们在肽合成和固定化方法、肽和 PTM 覆盖范围以及 PTM 组合潜力方面存在差异。此外，还描述了组蛋白 PTM 肽阵列的一些特殊应用，如定制阵列或双肽阵列。