Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), University of Cantabria, Santander, Spain.
Biotechnology and Cell Signaling, CNRS UMR7242, University of Strasbourg, Illkirch Cedex, France.
Methods Mol Biol. 2022;2529:171-206. doi: 10.1007/978-1-0716-2481-4_9.
The development of specific anti-modification antibodies as research tools has revolutionized the way histone methylation is studied. Lack of stringent quality controls, however, led to the development of nonspecific antibodies, compromising their use. In this chapter, we provide a series of protocols that collectively will help those studying histone methylation to develop and thoroughly validate high-end sequence-specific and methylation-dependent antibodies.
特异性抗修饰抗体作为研究工具的发展彻底改变了组蛋白甲基化的研究方式。然而，由于缺乏严格的质量控制，导致了非特异性抗体的产生，从而影响了它们的使用。在本章中，我们提供了一系列的方案，这些方案将共同帮助那些研究组蛋白甲基化的人开发和彻底验证高端的序列特异性和甲基化依赖性抗体。
