青春期接触香烟烟雾提取物会损害雄性小鼠的成年精子发生。
Puberty exposure to cigarette smoke extract impairs adult spermatogenesis in the mouse.
机构信息
Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, No. 63 Duobao Rd, Guangzhou, 510150, China; Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, No. 63 Duobao Rd, Guangzhou, 510150, China.
Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Rd, Guangzhou, 510080, China.
出版信息
Reprod Toxicol. 2019 Jan;83:8-13. doi: 10.1016/j.reprotox.2018.10.012. Epub 2018 Nov 2.
Epidemiological studies suggested that exposure to cigarette smoke early in life is associated with long-term health risks. Using a nuclear factor erythroid 2-related factor 2 (Nrf2) knockout (KO) mouse model, we found the sperm concentration and sperm motility were significantly lower in mice exposed to cigarette smoke extract (CSE) beginning at 4 weeks of age compared to 8 week-old mice. Additionally, CSE exposure significantly decreased in vitro fertilization (IVF) and blastocyst rates in the Nrf2 KO mice compared to the wild-type (WT) mice. Exposure to CSE resulted in the highest expression of the antioxidant genes in the CSE-treated WT mice at 8 weeks of age, and it was the lowest in the CSE-treated KO mice at 4 weeks. The study concluded that puberty exposure to CSE significantly affects adult spermatogenesis in agreement with the antioxidant Nrf2 genotype.
流行病学研究表明，生命早期接触香烟烟雾与长期健康风险有关。使用核因子红细胞 2 相关因子 2 (Nrf2) 敲除 (KO) 小鼠模型，我们发现与 8 周龄小鼠相比，从 4 周龄开始接触香烟烟雾提取物 (CSE) 的小鼠的精子浓度和精子活力明显降低。此外，与野生型 (WT) 小鼠相比，Nrf2 KO 小鼠的体外受精 (IVF) 和囊胚率显著降低。暴露于 CSE 导致在 8 周龄的 WT 小鼠中抗氧化基因的表达最高，而在 4 周龄的 KO 小鼠中表达最低。该研究得出的结论是，青春期接触 CSE 会显著影响成年精子发生，这与抗氧化 Nrf2 基因型一致。
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