Leszczynski D, Laszczynska M, Halttunen J, Häyry P
Kidney Int. 1987 Jun;31(6):1311-6. doi: 10.1038/ki.1987.144.
With an aim to investigate the relative sensitivity of various renal structures to allograft rejection, we analyzed the histochemical reaction intensity of seven enzymes prominently displayed in various rat kidney components, and correlated the expression of these enzymes both to the degree of intra-graft inflammation and to the expression of class II MHC antigens in graft capillary endothelial cells. Syngeneic transplants and normal renal tissue were used as controls. At the peak of inflammation, on the fifth day after transplantation, adenosine triphosphatase activity of vascular endothelial cells was strongly reduced in the peritubular capillary endothelium of the allograft, moderately in the glomerular endothelium but very little in the endothelium of arteries and veins. Lactate dehydrogenase, succinate dehydrogenase, isocitrate dehydrogenase, alkaline phosphatase, acid phosphatase and glucose-6-phosphatase activities were moderately reduced in the proximal tubular cells of the allograft and even less in the distal tubular cells. The results suggest that the prime target of the host immune attack is the intertubular capillary endothelium, whereas the distal tubular cells are relatively insensitive to immune injury.
为了研究各种肾结构对同种异体移植排斥反应的相对敏感性，我们分析了在各种大鼠肾脏成分中显著显示的七种酶的组织化学反应强度，并将这些酶的表达与移植物内炎症程度以及移植物毛细血管内皮细胞中II类MHC抗原的表达相关联。同基因移植和正常肾组织用作对照。在炎症高峰期，即移植后第五天，同种异体移植物的肾小管周围毛细血管内皮中血管内皮细胞的三磷酸腺苷酶活性强烈降低，肾小球内皮中中度降低，而动脉和静脉内皮中则很少降低。乳酸脱氢酶、琥珀酸脱氢酶、异柠檬酸脱氢酶、碱性磷酸酶、酸性磷酸酶和葡萄糖-6-磷酸酶活性在同种异体移植物的近端肾小管细胞中中度降低，在远端肾小管细胞中降低程度更小。结果表明，宿主免疫攻击的主要靶标是肾小管间毛细血管内皮，而远端肾小管细胞对免疫损伤相对不敏感。
