Department of Dermatology, Wuhan No. 1 Hospital, Wuhan, Hubei, China (mainland).
Med Sci Monit. 2018 Nov 5;24:7909-7913. doi: 10.12659/MSM.911347.
BACKGROUND NLPR3 is an important gene that belongs to the family of NOD-like receptors and is thought to play an important role in psoriasis. The aim of the present study was to investigate the expression of NLRP3 in psoriasis biopsy samples and to assess the possible correlation of its expression with that of interleukin IL-1β and Caspase-1. MATERIAL AND METHODS The mRNA expression was checked by qRT-PCR. The expression of the proteins was checked by Western blotting. The distribution of the proteins was determined by immunohistochemistry. RESULTS The results of our study indicate that the expression of NLRP3 was significantly upregulated in all the psoriatic biopsy samples as indicated by qRT-PCR and Western blotting. The expression of NLRP3 in psoriatic samples was 3.5 to 4.3 times higher than the expression of NLRP3 in normal skin biopsy samples. Moreover, our results indicated that the expression levels of IL-1β were higher as compared to the normal skin biopsy samples. Relative to the expression of IL-1β in normal skin biopsy samples, the expression of IL-1b was about 2.7-4.6 times higher. Additionally, the expression of caspase-1 was considerably upregulated in the psoriatic samples. Caspase-1 gene expression was 2.2-3.4 times higher than in normal skin biopsy samples. CONCLUSIONS NLPR3 may prove to be an important therapeutic target for psoriasis.
背景 NLRP3 是一种重要的基因，属于 NOD 样受体家族，被认为在银屑病中发挥重要作用。本研究旨在探讨 NLRP3 在银屑病活检样本中的表达，并评估其表达与白细胞介素（IL）-1β和 Caspase-1 表达的可能相关性。
材料与方法 通过 qRT-PCR 检查 mRNA 表达。通过 Western blot 检查蛋白质表达。通过免疫组织化学测定蛋白质的分布。
结果 我们的研究结果表明，NLRP3 在所有银屑病活检样本中的表达均显著上调，qRT-PCR 和 Western blot 均证实了这一点。NLRP3 在银屑病样本中的表达比正常皮肤活检样本中的表达高 3.5 至 4.3 倍。此外，我们的结果表明，IL-1β 的表达水平高于正常皮肤活检样本。与正常皮肤活检样本中的 IL-1β 表达相比，IL-1β 的表达高约 2.7-4.6 倍。此外，Caspase-1 在银屑病样本中的表达显著上调。Caspase-1 基因的表达比正常皮肤活检样本高 2.2-3.4 倍。
结论 NLRP3 可能成为治疗银屑病的重要靶点。
