Evtushenkov A N, Shevchik V E, Fomichev Iu K
Mol Gen Mikrobiol Virusol. 1987 May(5):22-5.
The gene for a pectate lyase of E. chrysanthemi ENA49 cloned in a recombinant plasmid pPTL1 (a derivative of RSF1010) was transferred into E. carotovora. The pectate lyase determined by the cloned gene was secreted into the cultural medium from the cells of E. crysanthemi EC16. Partial secretion of the enzyme was registered for E. carotovora cells. The major part of EC1 E. chrysanthemi pectate lyase synthesized by E. carotovora cells is accumulated in periplasmic and cytoplasmic fractions. The obtained results suggest the different specificity or efficiency of pectate lyase secretion systems in the studied Erwinia strains.
克隆于重组质粒pPTL1（RSF1010的衍生物）中的菊欧氏杆菌ENA49的果胶酸裂解酶基因被转入胡萝卜软腐欧文氏菌。由克隆基因所决定的果胶酸裂解酶从菊欧氏杆菌EC16细胞分泌到培养基中。胡萝卜软腐欧文氏菌细胞出现了该酶的部分分泌。胡萝卜软腐欧文氏菌细胞合成的菊欧氏杆菌EC1果胶酸裂解酶的大部分积累在周质和细胞质部分。所得结果表明在所研究的欧文氏菌菌株中果胶酸裂解酶分泌系统具有不同的特异性或效率。
