Lundh B, Kristensson K, Norrby E
Neuropathol Appl Neurobiol. 1987 Mar-Apr;13(2):111-22. doi: 10.1111/j.1365-2990.1987.tb00175.x.
Following intranasal instillation of vesicular stomatitis virus (VSV) in mice there was an extensive infection of the olfactory epithelium in contrast to a minimal involvement of the respiratory epithelium. Sendai virus (SV), on the other hand, caused an extensive infection of the respiratory epithelium and only minimal infection of the olfactory mucous membrane. VSV budded from basolateral surfaces of supporting cells and olfactory neurons, but not from their apical surfaces or the ciliated bulbous endings of the olfactory neuron dendrites. This asymmetric release of VSV favoured neuroinvasion. The virus spread along the olfactory nerves to the glomeruli in the olfactory bulbs after which it propagated transneuronally into the rest of the brain. SV budded only from the apical surface of respiratory epithelial cells, was released into the air passages, and there were no signs of invasion into the olfactory bulbs. Inoculation of the olfactory mucous membrane is a useful procedure for studies on selectivity of attack on peripheral neurons by viruses and on mechanisms of virus invasion of the nervous system in vivo.
给小鼠经鼻内接种水泡性口炎病毒（VSV）后，嗅上皮发生广泛感染，相比之下，呼吸道上皮受累程度极小。另一方面，仙台病毒（SV）引起呼吸道上皮广泛感染，而嗅黏膜仅有轻微感染。VSV从支持细胞和嗅神经元的基底外侧表面出芽，但不从它们的顶端表面或嗅神经元树突的纤毛球状末端出芽。VSV的这种不对称释放有利于神经侵袭。病毒沿着嗅神经传播到嗅球中的肾小球，之后经神经元传播到大脑的其他部位。SV仅从呼吸道上皮细胞的顶端表面出芽，释放到气道中，没有侵入嗅球的迹象。接种嗅黏膜对于研究病毒对周围神经元攻击的选择性以及病毒在体内侵袭神经系统的机制是一种有用的方法。
