Ko So-Ra, Lee Young-Ki, Srivastava Ankita, Park Seung-Hwan, Ahn Chi-Yong, Oh Hee-Mock
Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
IPst Company, Daejeon 35209, Republic of Korea.
J Microbiol Biotechnol. 2019 Jan 28;29(1):59-65. doi: 10.4014/jmb.1804.04031.
Fusaricidin analogs, produced by , were tested for selective control of a major bloom-forming cyanobacterium, sp. Fusaricidin (A and B mixtures) and four analogs were isolated from E681 and investigated for their inhibition of cyanobacterial cell growth. Among the four fusaricidin analogs, fraction 915 Da (designated as Fus901) showed growth inhibition activity for but not for and . Microcystin concentration decreased up to 70% and its content per cell also decreased over 50% after 3 days. Fusaricidin exhibited growth inhibition against Gram-positive bacteria but Fus901 did not. Molecular weights of fusaricidin A and B were 883 Da and 897 Da, whereas that of Fus901 was 915 Da. Structure analysis by a ring-opening method revealed a linear form for Fus901. Expression of the gene related to oxidative stress was increased 2.1-fold by Fus901 and that of decreased up to 40%. These results indicate that Fus901 exerts oxidative stress against . Thus, Fus901 can be used as a selective cyanobactericide without disturbing the ecological system and could help in decreasing the microcystin concentration.
由[未提及具体产生菌]产生的杀镰孢菌素类似物，针对一种主要的形成水华的蓝藻[未提及具体蓝藻种类]进行了选择性控制测试。从[未提及具体菌株]E681中分离出杀镰孢菌素（A和B混合物）及四种类似物，并研究了它们对蓝藻细胞生长的抑制作用。在这四种杀镰孢菌素类似物中，915 Da组分（命名为Fus901）对[未提及具体蓝藻种类]显示出生长抑制活性，但对[未提及具体蓝藻种类]和[未提及具体蓝藻种类]没有作用。3天后，微囊藻毒素浓度降低了70%，且每个细胞中的含量也降低了50%以上。杀镰孢菌素对革兰氏阳性菌有生长抑制作用，但Fus901没有。杀镰孢菌素A和B的分子量分别为883 Da和897 Da，而Fus901的分子量为915 Da。通过开环法进行的结构分析表明Fus901呈线性形式。与氧化应激相关的[未提及具体基因]基因的表达因Fus901而增加了2.1倍，而[未提及具体基因]的表达降低了40%。这些结果表明Fus901对[未提及具体蓝藻种类]施加了氧化应激。因此，Fus901可作为一种选择性杀藻剂，而不会干扰生态系统，并有助于降低微囊藻毒素浓度。
