Ali Sajid, Park Soon-Ki, Kim Won-Chan
School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
J Microbiol Biotechnol. 2018 Dec 28;28(12):1955-1970. doi: 10.4014/jmb.1808.08029.
Several genetic strategies have been proposed for the successful transformation and expression of microbial transgenes in model and crop plants. Here, we bring into focus the prominent applications of microbial transgenes in plants for the development of disease resistance; mitigation of stress conditions; augmentation of food quality; and use of plants as "" for the production of recombinant proteins, industrially important enzymes, vaccines, antimicrobial compounds, and other valuable secondary metabolites. We discuss the applicable and cost-effective approaches of transgenesis in different plants, as well as the limitations thereof. We subsequently present the contemporary developments in targeted genome editing systems that have facilitated the process of genetic modification and manifested stable and consumer-friendly genetically modified plants and their products. Finally, this article presents the different approaches and demonstrates the introduction and expression of microbial transgenes for the improvement of plant resistance to pathogens and abiotic stress conditions and the production of valuable compounds, together with the promising research progress in targeted genome editing technology. We include a special discussion on the highly efficient CRISPR- system helpful in microbial transgene editing in plants.
为了在模式植物和作物中成功转化和表达微生物转基因,人们提出了几种遗传策略。在这里,我们重点关注微生物转基因在植物中的突出应用,包括抗病性的发展;缓解胁迫条件;提高食品质量;以及利用植物作为生产重组蛋白、工业上重要的酶、疫苗、抗菌化合物和其他有价值的次生代谢物的“工厂”。我们讨论了在不同植物中转基因的适用且经济高效的方法及其局限性。随后,我们介绍了靶向基因组编辑系统的当代发展,这些发展促进了基因修饰过程,并产生了稳定且受消费者欢迎的转基因植物及其产品。最后,本文介绍了不同的方法,并展示了微生物转基因的导入和表达,以提高植物对病原体和非生物胁迫条件的抗性以及生产有价值的化合物,同时还介绍了靶向基因组编辑技术的 promising 研究进展。我们特别讨论了有助于植物中微生物转基因编辑的高效 CRISPR - 系统。