基因特征分析揭示 Neosartorin 生物合成机制，为杂二聚体天然产物的生成提供新视角。
Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation.
机构信息
Department of Biotechnology and Biomedicine , Technical University of Denmark , Søltofts Plads, 2800 Kongens Lyngby , Denmark.
Department of Chemistry , City University of Hong Kong , 83 Tat Chee Avenue , Kowloon , Hong Kong SAR, China.
出版信息
Org Lett. 2018 Nov 16;20(22):7197-7200. doi: 10.1021/acs.orglett.8b03123. Epub 2018 Nov 5.
A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.
在新型烟曲霉中发现了真菌黄烷酮杂二聚体neosartorin（1）的生物合成基因簇，并通过一系列基因缺失实验对其生物合成进行了研究。结果表明，1 的两个单体单元由同一套酶合成，其中大黄酚（5）为共同前体。此外，还发现了用于杂二聚化的 P450 单加氧酶 NsrP，它还接受非天然底物，从而提供新的黄烷酮二聚体。
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