Gaevaia L V, Zhukov V N
Prikl Biokhim Mikrobiol. 1987 May-Jun;23(3):309-16.
Nucleoside phosphorylases from E. coli immobilized in polyacrylamide gel were used for production of nucleosides from nitrous bases during the transglucosylase reaction. Phosphorylation of nucleosides resulted in the formation of 5'-nucleotides was performed in the presence of carrot nucleoside phosphotransferase immobilized in polyacrylamide gel. Using 14C-labelled nitrous bases as starting substrates, labelled nucleosides and nucleotides can be obtained with the 75-80% yield that have radioactive purity of 95-99%. The stability of the immobilized enzymes was being studied under the conditions of the reactions using them in the batch and plug flow reactors.
固定在聚丙烯酰胺凝胶中的大肠杆菌核苷磷酸化酶用于在转葡糖基酶反应过程中由含氮碱基生产核苷。核苷的磷酸化导致在固定于聚丙烯酰胺凝胶中的胡萝卜核苷磷酸转移酶存在下形成5'-核苷酸。使用14C标记的含氮碱基作为起始底物，可以获得放射性纯度为95-99%、产率为75-80%的标记核苷和核苷酸。在分批和活塞流反应器中使用固定化酶的反应条件下研究了它们的稳定性。
