多能干细胞标志物足细胞粘附蛋白在前列腺癌中的过表达
Overexpression of the Pluripotent Stem Cell Marker Podocalyxin in Prostate Cancer.
作者信息
Heath Elisabeth I, Heilbrun Lance K, Smith Daryn, Schopperle William M, Ju Yawen, Bolton Susan, Ahmed Quratulain, Sakr Wael A
机构信息
Barbara Ann Karmanos Cancer Institute, Department of Oncology, Wayne State University School of Medicine, Detroit, MI, U.S.A.
CureMeta LLC, Boston, MA, U.S.A.
出版信息
Anticancer Res. 2018 Nov;38(11):6361-6366. doi: 10.21873/anticanres.12994.
BACKGROUND/AIM: Podocalyxin, a member of the CD34 family of cell surface sialomucins, is overexpressed in human embryonal carcinoma cell lines, as well as in several cancer types, and is associated with poor prognosis. Podocalyxin variants are associated with an increased risk and aggressiveness of prostate cancer. Herein podocalyxin protein expression in prostate cancer was characterized.
MATERIALS AND METHODS
Expression of podocalyxin as well as of TRA-1-60 and TRA-1-81 antigens was assessed immunohistochemically in 84 radical prostatectomy specimens and in adjacent normal tissues.
RESULTS
Podocalyxin expression and H-scores were considerably higher in prostate tumors compared to normal tissues. High TRA-1-60 and TRA-1-81 staining was detected, however, in a much smaller percentage of prostate tumors, while their expression and H-scores were low in normal tissues. Similar trends for all three proteins were observed in prostatic intraepithelial neoplasia.
CONCLUSION
Overexpression of podocalyxin in prostate cancer renders the protein a putative immunohistochemical marker of prostate cancer that may contribute to stratification of patients for optimal treatment.
背景/目的：足细胞毒素是细胞表面唾液酸黏蛋白CD34家族的成员之一，在人胚胎癌细胞系以及多种癌症类型中过表达，且与预后不良相关。足细胞毒素变体与前列腺癌风险增加及侵袭性增强有关。本文对前列腺癌中足细胞毒素蛋白表达进行了表征。
材料与方法
采用免疫组织化学方法评估了84例前列腺癌根治术标本及相邻正常组织中足细胞毒素以及TRA-1-60和TRA-1-81抗原的表达。
结果
与正常组织相比，前列腺肿瘤中足细胞毒素表达及H评分显著更高。然而，在比例小得多的前列腺肿瘤中检测到高TRA-1-60和TRA-1-81染色，而它们在正常组织中的表达及H评分较低。在前列腺上皮内瘤变中观察到这三种蛋白的相似趋势。
结论
足细胞毒素在前列腺癌中的过表达使该蛋白成为前列腺癌一种可能的免疫组化标志物，这可能有助于对患者进行分层以实现最佳治疗。
Zotero 插件