Rock E, Kozak-Reiss G
Arch Biochem Biophys. 1987 Aug 1;256(2):703-7. doi: 10.1016/0003-9861(87)90628-x.
Trapezius muscle from normal and malignant hyperthermia (MH) pigs was used to investigate the effects of halothane on contractile properties and on the calcium transport system of isolated surface membranes. We observed that (i) halothane, diluted in dimethyl sulfoxide, induced a higher isometric contracture response in MH muscle than in normal muscle, (ii) halothane had a more pronounced inhibitory effect on the sarcolemmal Ca2+-ATPase activity in MH membrane, and (iii) the actively accumulated calcium was released in higher amounts in MH muscle than in normal muscle. These results suggest that halothane might induce, in vivo, an important influx of extracellular calcium ions through the MH sarcolemmal membranes and this pool of intracellular calcium may constitute the trigger for the defective sarcoplasmic reticulum "calcium-induced calcium-release" system.
使用来自正常猪和恶性高热（MH）猪的斜方肌来研究氟烷对离体表面膜收缩特性和钙转运系统的影响。我们观察到：（i）在二甲亚砜中稀释的氟烷在MH肌肉中比在正常肌肉中诱导出更高的等长挛缩反应；（ii）氟烷对MH膜中肌膜Ca2 + -ATP酶活性具有更显著的抑制作用；（iii）在MH肌肉中比在正常肌肉中释放出更多量的主动积累的钙。这些结果表明，氟烷在体内可能通过MH肌膜诱导细胞外钙离子大量内流，并且这一细胞内钙库可能构成有缺陷的肌浆网“钙诱导钙释放”系统的触发因素。
