Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
College of Environment and Resources, Jilin University, Changchun, 130021, China.
Water Res. 2019 Jan 1;148:398-406. doi: 10.1016/j.watres.2018.10.073. Epub 2018 Oct 30.
Understanding the fate of overall antibiotic resistance genes (ARGs) during the biological treatment of antibiotic containing wastewater is a central issue for the water ecological safety assessment. Although the microbial electrode-respiration based biotransformation process could significantly detoxify some antibiotic contaminants, e.g. chloramphenicol (CAP), the response of CAP-reducing biocathode microbiome and resistome to continuous electrical stimulation, especially ARGs network interactions, are poorly understood. Here, using highthroughput functional gene array (GeoChip v4.6) and Illumina 16S rRNA gene MiSeq sequencing, the structure, composition, diversity and network interactions of CAP-reducing biocathode microbiome and resistome in response to continuous electrical stimulation were investigated. Our results indicate that the CAP bioelectroreduction process could significantly accelerate the elimination of antibacterial activity of CAP during CAP-containing wastewater treatment compared to the pure bioreduction process. Continuous electrical stimulation could obviously alter both the microbiome and resistome structures and consistently decrease the phylogenetic, functional and overall ARGs diversity and network complexity within the CAP-reducing biofilms. The relative abundances of overall ARGs and specific CAP resistance related major facilitator superfamily (MFS) transporter genes were significantly negatively correlated with the reduction efficiency of CAP to inactive antibacterial product AMCl (partially dechlorinated aromatic amine), which may reduce the ecological risk associated with the evolution of multidrug-resistant bacteria and ARGs during antibiotic-containing wastewater treatment process. This study offers new insights into the response of an antibiotic reducing biocathode resistome to continuous electrical stimulation and provides useful information on the assessment of overall ARGs risk for the bioelectrochemical treatment of antibiotic contaminants.
理解含抗生素废水生物处理过程中抗生素抗性基因（ARGs）的命运是评估水生态安全的核心问题。虽然基于微生物电极呼吸的生物转化过程可以显著解毒一些抗生素污染物，如氯霉素（CAP），但 CAP 还原生物阴极微生物组和抗性组对连续电刺激的响应，尤其是 ARGs 网络相互作用，还知之甚少。在这里，我们使用高通量功能基因芯片（GeoChip v4.6）和 Illumina 16S rRNA 基因 MiSeq 测序，研究了连续电刺激下 CAP 还原生物阴极微生物组和抗性组的结构、组成、多样性和网络相互作用。我们的结果表明，与纯生物还原过程相比，含 CAP 废水处理过程中的 CAP 生物电化学还原过程可以显著加速 CAP 抗菌活性的消除。连续电刺激可以明显改变微生物组和抗性组的结构，并一致降低 CAP 还原生物膜内的系统发育、功能和整体 ARGs 多样性和网络复杂性。整体 ARGs 和特定 CAP 抗性相关的主要易化因子超家族（MFS）转运基因的相对丰度与 CAP 向无活性抗菌产物 AMCl（部分脱氯芳族胺）的还原效率呈显著负相关，这可能会降低与多药耐药菌和 ARGs 进化相关的生态风险抗生素废水处理过程中。本研究深入了解了抗生素还原生物阴极抗性组对连续电刺激的响应，并为评估抗生素污染物的生物电化学处理过程中整体 ARGs 风险提供了有用信息。
