Nair N, Kurup C K
Biochim Biophys Acta. 1987 Sep 11;925(3):332-40. doi: 10.1016/0304-4165(87)90199-1.
Administration of the widely used plasticizer di(2-ethylhexyl)phthalate (2% w/w) in the diet to the rat caused proliferation of mitochondria in the liver. The number of mitochondria as well as the amount of protein recovered in the organellar fraction was doubled. Mitochondria isolated from the livers of treated animals showed decreased (50%) respiratory activity. The content and activity of cytochrome oxidase were also decreased. The specific incorporation of amino acids into the proteins of whole liver and of mitochondria was not increased in plasticizer-treated animals. Isolated mitochondria also did not show any difference in the rate of incorporation of amino acids into proteins. The half-lives of whole liver proteins and of mitochondria were increased in plasticizer-fed animals. The half-life of cytochrome oxidase, however, was unaffected by the treatment. The pattern of double labeling of mitochondrial proteins confirmed decreased turnover in plasticizer-treated animals.
在大鼠饮食中添加广泛使用的增塑剂邻苯二甲酸二（2-乙基己基）酯（2% w/w）会导致肝脏中线粒体增殖。线粒体数量以及从细胞器部分回收的蛋白质量增加了一倍。从经处理动物肝脏中分离出的线粒体显示呼吸活性降低（50%）。细胞色素氧化酶的含量和活性也降低了。在经增塑剂处理的动物中，氨基酸掺入全肝和线粒体蛋白中的特异性并未增加。分离出的线粒体在氨基酸掺入蛋白的速率方面也未显示出任何差异。在喂食增塑剂的动物中，全肝蛋白和线粒体的半衰期延长。然而，细胞色素氧化酶的半衰期不受该处理的影响。线粒体蛋白双标记模式证实了经增塑剂处理的动物中蛋白周转减慢。
