Ramis G, Martínez-Alarcón L, Medina-Moreno E, Abellaneda J M, Quereda J J, Febrero B, Sáez-Acosta A, Ríos A, Muñoz A, Ramírez P, Majado M J
Departamento de Producción Animal, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain; Instituto Murciano de Investigaciones Biosanitaria, Murcia, Spain.
Instituto Murciano de Investigaciones Biosanitaria, Murcia, Spain; Servicio de Cirugía, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
Transplant Proc. 2018 Nov;50(9):2842-2846. doi: 10.1016/j.transproceed.2018.03.048. Epub 2018 Mar 15.
The immunorejection in xenotransplantation has mostly been studied from the host's immune system activation point of view and there is very little information about the graft-vs-host reaction.
To validate an enzyme-linked immunosorbent assay (ELISA) test for porcine IgM and IgG quantitation, the assessment of porcine IgG and IgM in sera samples from baboons after liver orthotopic xenotransplantation or in human plasma after xenotransfusion through pig organs, and to assess the presence of porcine immunoglobulin in a baboon after plasmapheresis to a complete change of plasma after 4 passages through pig liver.
Two commercial ELISA kits for pig IgG and IgM quantitation were evaluated for cross reactivity with samples from baboons, Rhesus monkeys, squirrel monkeys, and humans. Then, samples from 18 baboons after orthotopic liver xenotransplantation were studied for porcine IgG and IgM. To understand the phenomenon, human plasma samples after xenotransfusion 1, 2, 3, or 4 times through liver or kidney were assessed for porcine IgG presence and finally, the porcine IgG were quantified in sera samples obtained during more than 4 years from a baboon after plasmapheresis with baboon plasma after xenotransfusion 4 times through a pig liver.
Porcine IgG and IgM were found in samples from xenotransplanted baboon during all survival. The quantity of porcine IgG in plasma after xenotransfusion correlated with the number of passages through the pig liver, and the IgG were completely cleared from the baboon 16 days after plasmapheresis and complete substitution of plasma after 4 xenotransfusions through a pig liver.
异种移植中的免疫排斥大多是从宿主免疫系统激活的角度进行研究的，而关于移植物抗宿主反应的信息非常少。
验证一种用于定量猪IgM和IgG的酶联免疫吸附测定（ELISA）试验，评估原位肝异种移植后狒狒血清样本中或通过猪器官进行异种输血后人血浆中的猪IgG和IgM，并评估狒狒在经过4次通过猪肝的血浆置换直至血浆完全更换后猪免疫球蛋白的存在情况。
评估了两种用于定量猪IgG和IgM的商用ELISA试剂盒与来自狒狒、恒河猴、松鼠猴和人类样本的交叉反应性。然后，研究了18只原位肝异种移植后狒狒的样本中的猪IgG和IgM。为了解这一现象，评估了通过肝脏或肾脏进行1、2、3或4次异种输血后人血浆样本中猪IgG的存在情况，最后，对一只狒狒在经过4次通过猪肝的异种输血后进行血浆置换并更换狒狒血浆后4年多时间内获得的血清样本中的猪IgG进行了定量。
在异种移植狒狒的所有存活期样本中均发现了猪IgG和IgM。异种输血后血浆中猪IgG的量与通过猪肝的次数相关，并且在血浆置换以及经过4次通过猪肝的异种输血后血浆完全置换16天后，狒狒体内的IgG被完全清除。
