Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Int J Biol Macromol. 2019 Feb 1;122:348-358. doi: 10.1016/j.ijbiomac.2018.10.179. Epub 2018 Oct 26.
An experiment was conducted to determine the effects of swollenin on the enzymatic hydrolysis and in vitro rumen microbial fermentation of agricultural straws. The swollenin from Trichoderma reesei was produced in Pichia pastoris in current study. The recombinant protein caused an obvious disruption on filter paper and slight increase in reducing sugars release when treating xylan, Avicel, rice straw, wheat straw, and corn straw. Simultaneous incubation of swollenin with fibrolytic enzyme resulted in a significant synergistic activity on disruption of filter paper and hydrolysis of above-mentioned substrates. During in vitro rumen fermentation of three straw diets, the dry matter digestibility, acetate production, microbial protein synthesis, and fibrolytic bacterial population were increased by swollenin. This study demonstrates that swollenin could enhance the enzymatic hydrolysis and in vitro ruminal fermentation of agricultural straws, showing the potential of swollenin for improving the utilization of agricultural straws in ruminants.
本研究在毕赤酵母中生产里氏木霉来源的膨胀素。该重组蛋白在处理木聚糖、Avicel、水稻秸秆、小麦秸秆和玉米秸秆时，明显破坏滤纸并轻微增加还原糖释放。膨胀素与纤维酶同时孵育对滤纸破坏和上述底物水解具有显著协同活性。在三种秸秆日粮的体外瘤胃发酵过程中，膨胀素增加了干物质消化率、乙酸产量、微生物蛋白合成和纤维分解菌的数量。本研究表明，膨胀素可以增强农业秸秆的酶解和体外瘤胃发酵，表明膨胀素在提高反刍动物对农业秸秆的利用方面具有潜力。
