Liaw K Y, Kuo L L, Chen C C, Lin-Shiau S Y
Circ Shock. 1987;22(3):195-203.
Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase activities of erythrocyte membrane, microsomal fractions of rectus muscle, and liver were measured colorimetrically in the biopsy specimens of 14 control, 7 uncomplicated trauma (group 2), and 14 severe trauma or septic patients (groups 3-A and 3-B). In erythrocytes, these three ATPase activities in group 2 were not significantly changed but sepsis of both the acute (group 3-A) and ongoing type (group 3-B) decreased all of the ATPase activities. In muscle, there was a significant loss of three ATPase activities in the acute insult of severe trauma or sepsis (group 3-A), while Na+-K+-ATPase and Mg2+-ATPase activities were not significantly changed in ongoing, severe trauma (group 3-B). In the liver, a tendency for all three ATPase activities to decrease is noted in the severe traumatic group. However, a statistical difference between the control and severe traumatic group showed only for Na+-K+ ATPase and Mg2+-ATPase in group 3-A and Ca2+-ATPase in group 3-B. Correlation coefficients between erythrocyte, muscle, and liver for three ATPase activities are between 0.4 and 0.5. The mechanism which alters ATPase activity remains unknown in this study, but it may account for the variation in traumatic insult, in hemodynamic and hormone changes, and in tissue energy stores.
采用比色法对14名对照者、7名无并发症创伤患者（第2组）以及14名严重创伤或脓毒症患者（第3 - A组和第3 - B组）的活检标本中红细胞膜、直肌微粒体部分和肝脏的钠钾ATP酶、钙ATP酶和镁ATP酶活性进行了测定。在红细胞中，第2组的这三种ATP酶活性无显著变化，但急性脓毒症（第3 - A组）和持续性脓毒症（第3 - B组）均使所有ATP酶活性降低。在肌肉中，严重创伤或脓毒症的急性损伤（第3 - A组）导致三种ATP酶活性显著丧失，而在持续性严重创伤（第3 - B组）中，钠钾ATP酶和镁ATP酶活性无显著变化。在肝脏中，严重创伤组的所有三种ATP酶活性均有降低趋势。然而，对照组与严重创伤组之间的统计学差异仅在第3 - A组的钠钾ATP酶和镁ATP酶以及第3 - B组的钙ATP酶中表现出来。红细胞、肌肉和肝脏中三种ATP酶活性的相关系数在0.4至0.5之间。本研究中改变ATP酶活性的机制尚不清楚，但它可能解释了创伤性损伤、血流动力学和激素变化以及组织能量储备的差异。
