Khalil F, Fine B, Kuriyama S, Hatori N, Nakamura A, Nakamura M, Aviv A
Clin Exp Hypertens A. 1987;9(4):741-52. doi: 10.3109/10641968709161447.
To explore the role of the atrial natriuretic factor (ANF) system in the pathophysiology of hypertension we examined the binding kinetics of synthetic ANF to cultured vascular smooth muscle cells (VSMCs) derived from the spontaneously hypertensive rat (SHR) and two normotensive controls-the Wistar Kyoto (WKY) and American Wistar (W). The number of maximal binding sites (Bmax) per cell (mean +/- SEM; X10(3] were: SHR = 278.0 +/- 33.0, WKY = 28.3 +/- 7.1 and W = 26.6 +/- 4.2. The differences between the SHR and normotensive strains were significant at p less than 0.001. The equilibrium dissociation constant (Kd; X 10(-9)M) was higher in SHR VSMCs (0.94 +/- 0.14) than in WKY (0.22 +/- 0.09; p less than 0.01) and W (0.39 +/- 0.14; p less than 0.02) cells. The plasma levels of the immunoreactive ANF were higher in SHR than the normotensive controls. We suggest that the relatively greater ANF receptor density in cultured VSMCs of the SHR represents a response to the in vitro environment which is relatively more deficient in ANF for VSMCs of the SHR as compared with the normotensive rats. Thus, the capacity of the SHR VSMC to regulate ANF receptor density appears to be independent of the blood pressure level.
为探讨心钠素（ANF）系统在高血压病理生理学中的作用，我们检测了合成的心钠素与源自自发性高血压大鼠（SHR）及两种正常血压对照大鼠——Wistar Kyoto（WKY）和美国Wistar（W）的培养血管平滑肌细胞（VSMC）的结合动力学。每个细胞的最大结合位点（Bmax）数量（平均值±标准误；×10³）分别为：SHR = 278.0±33.0，WKY = 28.3±7.1，W = 26.6±4.2。SHR与正常血压品系之间的差异在p<0.001时具有显著性。SHR的血管平滑肌细胞的平衡解离常数（Kd；×10⁻⁹M）（0.94±0.14）高于WKY（0.22±0.09；p<0.01）和W（0.39±0.14；p<0.02）细胞。SHR的免疫反应性心钠素的血浆水平高于正常血压对照。我们认为，与正常血压大鼠相比，SHR培养的血管平滑肌细胞中相对较高的心钠素受体密度代表了对体外环境的一种反应，该环境对SHR的血管平滑肌细胞而言心钠素相对缺乏。因此，SHR血管平滑肌细胞调节心钠素受体密度的能力似乎与血压水平无关。
