正常和淋巴细胞增多绵羊的外周血T淋巴细胞和B淋巴细胞

T and B peripheral blood lymphocytes in normal and lymphocytotic sheep.

作者信息

Djilali S, Parodi A L

出版信息

Comp Immunol Microbiol Infect Dis. 1987;10(2):141-7. doi: 10.1016/0147-9571(87)90008-7.

Abstract

Surface immunoglobulins (SIg), Peanut Agglutinin (PNA), spontaneous erythrocyte rosette (E-rosette) and Helix pomatia (HP) marker were investigated in normal and Bovine leukemia virus (BLV)-infected sheep. In normal sheep, 19.3% +/- 4.9 of peripheral blood lymphocytes (PBL) were SIg+, whereas 58% +/- 5.69 were PNA+, and 19.6 +/- 5.2 were E-rosette forming cells (E-RFC). In BLV-induced lymphocytotic sheep, SIg+ cells in PBL reached 59.4% +/- 15.06. In the same animals, PNA bound to 20.6% +/- 9.69 and E-RFC were 8.7% +/- 4.5. A panning technique was applied with an anti sheep-immunoglobulins coated plates to separate SIg+ (adherent cells = A) and SIg- cells (non-adherent cells = NA). The (A) population was 94-95% SIg+ cells and 2-3% PNA+, while the (NA) population was 0-4% SIg+ and 79-85% PNA+ cells. Thus PNA is a T cell marker in sheep species. HP, a marker for bovine T lymphocytes was also studied. Sheep PBL do not bind to HP. However, after panning separation about 50% of NA cells became HP+.

摘要

对正常和感染牛白血病病毒（BLV）的绵羊进行了表面免疫球蛋白（SIg）、花生凝集素（PNA）、自发红细胞花环（E花环）和苹果蜗牛（HP）标记物的研究。在正常绵羊中，外周血淋巴细胞（PBL）中19.3%±4.9%为SIg阳性，而58%±5.69%为PNA阳性，19.6±5.2为E花环形成细胞（E-RFC）。在BLV诱导的淋巴细胞增多的绵羊中，PBL中的SIg阳性细胞达到59.4%±15.06%。在同一批动物中，PNA结合率为20.6%±9.69%，E-RFC为8.7%±4.5%。采用抗绵羊免疫球蛋白包被板的淘选技术分离SIg阳性（贴壁细胞=A）和SIg阴性细胞（非贴壁细胞=NA）。（A）群体中94 - 95%为SIg阳性细胞，2 - 3%为PNA阳性，而（NA）群体中0 - 4%为SIg阳性，79 - 85%为PNA阳性细胞。因此，PNA是绵羊物种中的T细胞标记物。还研究了作为牛T淋巴细胞标记物的HP。绵羊PBL不与HP结合。然而，淘选分离后，约50%的NA细胞变为HP阳性。