Naruto Research Institute, Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima, Japan.
J Med Microbiol. 2018 Dec;67(12):1796-1803. doi: 10.1099/jmm.0.000870. Epub 2018 Nov 7.
We assessed the fast-acting bactericidal activity and substantivity of olanexidine gluconate (OLG) to investigate its remaining bactericidal activity on the skin after rinsing and drying by using an ex vivo Yucatan micropig (YMP) skin model.
The fast-acting bactericidal activity was evaluated in pigskin models inoculated with methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, vancomycin-resistant Enterococcus faecalis (VRE), Acinetobacter baumannii, Corynebacterium minutissimum and Cutibacterium acnes. To evaluate substantivity, the YMP skin piece first had 1.5 % OLG, chlorhexidine gluconate (CHG) formulations or 10 % povidone-iodine (PVP-I) applied to it, and was then rinsed with distilled water, incubated for 4, 6, 8 or 12 h and inoculated with the test bacteria (MRSA, S. epidermidis and VRE). The viable bacteria remaining at 1 min of exposure of bacteria were counted to measure the quantity of antiseptic molecules retaining bactericidal activity. To determine the factors contributing to the substantivity, the stratum corneum (SC) of the YMP skin that had had OLG or CHG applied to it was exfoliated using a tape-stripping method and the amount of antiseptic was quantitated.
OLG showed a fast-acting bactericidal activity that was similar to or stronger than that of CHG formulations up to a concentration of 1 % and PVP-I with a short exposure time of 30 s, and substantivity until 12 h after rinsing, whereas the other antiseptics hardly showed any substantivity. There was 2.8 times or more OLG in the SC than CHG.
OLG has fast-acting activity and substantivity, which are required properties for an antiseptic, and is useful for preventing infections.
我们评估了葡萄糖酸奥替尼啶（OLG）的快速杀菌活性和持效性，以使用离体尤卡坦微型猪（YMP）皮肤模型研究其在冲洗和干燥后皮肤表面的残留杀菌活性。
在接种耐甲氧西林金黄色葡萄球菌（MRSA）、表皮葡萄球菌、万古霉素耐药粪肠球菌（VRE）、鲍曼不动杆菌、极小棒状杆菌和痤疮丙酸杆菌的猪皮模型中评估快速杀菌活性。为了评估持效性，首先将 YMP 皮肤片涂抹 1.5%的 OLG、葡萄糖酸洗必泰（CHG）制剂或 10%聚维酮碘（PVP-I），然后用蒸馏水冲洗，孵育 4、6、8 或 12 小时，并用测试细菌（MRSA、表皮葡萄球菌和 VRE）接种。在细菌暴露 1 分钟时计数残留的活菌，以测量保留杀菌活性的消毒剂分子的数量。为了确定导致持效性的因素，使用胶带剥离法从涂抹了 OLG 或 CHG 的 YMP 皮肤的角质层（SC）中剥落，并定量消毒剂的量。
OLG 表现出快速杀菌活性，其杀菌活性与 1%浓度的 CHG 制剂或 30 秒短暴露时间的 PVP-I 相当或更强，并且在冲洗后 12 小时内具有持效性，而其他消毒剂几乎没有持效性。SC 中的 OLG 是 CHG 的 2.8 倍以上。
OLG 具有快速作用活性和持效性，这是消毒剂的必需特性，对预防感染很有用。
