Beomidehagh Mahsa, Rezaee Mohammad Ahangarzadeh, Ganbarov Khudaverdi, Jafari Farnaz, Hasani Alka, Alizadeh Naser, Tanomand Asghar, Kafil Hossein Samadi
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Cell Mol Biol (Noisy-le-grand). 2018 Oct 30;64(13):1-5.
Enterococcus faecalis is an important opportunistic infectious agent involving the oral cavity and endodontics. The aim of this study was to evaluate the expression ratio of efaA gene in biofilm producer E. faecalis before and after receiving acidic and alkali shocks. One hundred E. faecalis isolates were gathered from 170 infectious root canals. After analysis of biofilm formation by the Microtiterplate method, the presence of efaA gene was examined by PCR and its expression was evaluated by Real-time PCR, one before applying any stressed to isolates and another by applying acidic and alkali shock. Chi-square method was used for statistical analysis. Eighty-two percent of samples had efaA gene. Evaluation of biofilm formation, 49% of the isolates were strong biofilm producer, 42% moderate and 10 % of them had no biofilm. 59% overexpression of efaA gene was observed in biofilm producer isolates, while there were no significant changes in samples with acidic stress and decreased expression after alkali shock. Findings of the present study, indicates importance of efaA gene in biofilm formation and pathogenesis of E. faecalis. Acid had no effect of expression of this gene but alkali reduced expression of this gene in a significant level. These results indicate the importance of efaA and acidic conditions in biofilm production by E. faecalis.
粪肠球菌是一种涉及口腔和牙髓病学的重要机会性感染病原体。本研究的目的是评估产生物膜的粪肠球菌在受到酸和碱冲击前后efaA基因的表达率。从170个感染根管中收集了100株粪肠球菌分离株。通过微量滴定板法分析生物膜形成后，通过PCR检测efaA基因的存在，并通过实时PCR评估其表达，一次是在对分离株施加任何应激之前，另一次是在施加酸和碱冲击之后。采用卡方检验进行统计分析。82%的样本含有efaA基因。对生物膜形成的评估显示，49%的分离株是强生物膜产生菌，42%为中度，10%不产生物膜。在产生物膜的分离株中观察到59%的efaA基因过表达，而在酸应激样本中没有显著变化，碱冲击后表达降低。本研究结果表明efaA基因在粪肠球菌生物膜形成和发病机制中的重要性。酸对该基因的表达没有影响，但碱在显著水平上降低了该基因的表达。这些结果表明efaA和酸性条件在粪肠球菌生物膜产生中的重要性。
