Cheng P Y, Yasuda H, Katori T, Oka H
Endocrinol Jpn. 1987 Apr;34(2):189-202. doi: 10.1507/endocrj1954.34.189.
The relationship between glucose-induced insulin secretion and metabolism of inositol phospholipid was investigated by means of an islet perifusion method and direct measuring of inositol phosphates after sonicating the islets. The results showed that the time course of inositol phospholipid breakdown is coincident with the first phase of glucose-induced insulin secretion. Analysis of the effluent perifusate as well as the water soluble inositol-containing substance after sonication of stimulated islets revealed that most of the metabolite of inositol phospholipid is inositol-triphosphate, the hydrolysis product of phosphatidylinositol-4,5-bisphosphate. On the other hand, perifusion of islets with exogenous inositol-triphosphate showed a monophasic and dose-dependent response of insulin secretion. Thus, the initial process of glucose stimulation is accompanied with the formation of inositol-triphosphate, which is a possible candidate for the triggering of first phase insulin secretion.
采用胰岛灌流法并在对胰岛进行超声处理后直接测定肌醇磷酸，研究了葡萄糖诱导的胰岛素分泌与肌醇磷脂代谢之间的关系。结果表明，肌醇磷脂分解的时间进程与葡萄糖诱导的胰岛素分泌的第一相相吻合。对受刺激胰岛超声处理后的流出灌流液以及水溶性含肌醇物质的分析表明，肌醇磷脂的大部分代谢产物是肌醇三磷酸，即磷脂酰肌醇-4,5-二磷酸的水解产物。另一方面，用外源性肌醇三磷酸灌流胰岛显示出胰岛素分泌的单相剂量依赖性反应。因此，葡萄糖刺激的初始过程伴随着肌醇三磷酸的形成，肌醇三磷酸可能是触发胰岛素分泌第一相的候选物质。
