在感染SV40和腺病毒后，G0特异性温度敏感突变体tsJT60中细胞DNA合成的诱导。

Induction of cellular DNA synthesis in G0-specific ts mutant, tsJT60, following infection with SV40 and adenoviruses.

作者信息

Ninomiya-Tsuji J, Goto Y, Ishibashi S, Shiroki K, Ide T

出版信息

Exp Cell Res. 1987 Aug;171(2):509-12. doi: 10.1016/0014-4827(87)90183-2.

DOI:10.1016/0014-4827(87)90183-2

Abstract

tsJT60 cells, a temperature-sensitive G0 mutant of a Fischer rat cell line, grew normally in an exponential growth phase at both permissive (34 degrees C) and nonpermissive (39.5 degrees C) temperatures, but when stimulated with fetal bovine serum in the growth-arrested state (G0 phase) they entered S phase at 34 degrees C but not at 39.5 degrees C. Infection of G0-arrested tsJT60 cells with SV40, adenovirus (Ad) 5 wild type and its E1B mutant dl313, and Ad12 wild type and its E1B mutants in205B, in205C, dl205, and in206B induced DNA synthesis at both temperatures. The DNA synthesized after virus infection was shown to be cellular by Hirt separation of DNA from SV40-infected cells and by CsCl equilibrium density gradient centrifugation of DNA from Ad5-infected cells.

摘要

tsJT60细胞是Fischer大鼠细胞系的一种温度敏感型G0突变体，在允许温度（34℃）和非允许温度（39.5℃）下的指数生长期均能正常生长，但在生长停滞状态（G0期）用胎牛血清刺激时，它们在34℃进入S期，而在39.5℃则不进入。用SV40、腺病毒（Ad）5野生型及其E1B突变体dl313，以及Ad12野生型及其E1B突变体in205B、in205C、dl205和in206B感染处于G0期停滞的tsJT60细胞，在两种温度下均诱导DNA合成。通过从SV40感染细胞中进行Hirt DNA分离，以及对Ad5感染细胞的DNA进行CsCl平衡密度梯度离心，证明病毒感染后合成的DNA是细胞性的。

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Entrance of SV40-transformed cells into G0 phase as revealed by a study using the G0-specific ts mutant tsJT60.使用G0特异性温度敏感突变体tsJT60的一项研究揭示，SV40转化细胞进入G0期。

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8

Bypass of the ts block of tsJT60, a G0-specific ts mutant from rat fibroblasts, by fetal bovine serum and epidermal growth factor.胎牛血清和表皮生长因子对tsJT60（一种来自大鼠成纤维细胞的G0期特异性温度敏感突变体）的温度敏感阻断的旁路作用

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