从GFAP-CreER转基因仔猪分离并鉴定GFAP阳性猪神经干细胞/祖细胞。

Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreER transgenic piglet.

作者信息

Kim Eunhye, Hwang Seon-Ung, Yoon Junchul David, Kim Hyunggee, Lee Gabsang, Hyun Sang-Hwan

机构信息

Laboratory of Veterinary Embryology and Biotechnology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, 1 Chungdae-ro, Seowon-gu, Cheongju, 28644, Republic of Korea.

Institute of Stem Cell & Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, 28644, Chungbuk, Republic of Korea.

出版信息

BMC Vet Res. 2018 Nov 7;14(1):331. doi: 10.1186/s12917-018-1660-4.

DOI:10.1186/s12917-018-1660-4

PMID:30404643

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6222979/

Abstract

BACKGROUND

The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model.

RESULTS

Here, we isolated GFAP-positive porcine neural stem cells (NSCs) from the brain explant of a transgenic piglet, with expression of CreER under the control of the GFAP promoter (pGFAP-CreER). The isolated pGFAP-CreER NSCs showed self-renewal and expression of representative NSC markers such as Nestin and Sox2. Pharmacological inhibition studies revealed that Notch1 signaling is necessary to maintain NSC identity, whereas serum treatment induced cell differentiation into reactive astrocytes and neurons.

CONCLUSIONS

Collectively, these results indicate that GFAP promoter-driven porcine CreER NSCs would be a useful tool to study neurogenesis of the porcine adult central nervous system and furthers our understanding of its potential clinical application in the future. ᅟ.

摘要

背景

猪脑是回脑型，其灰质和白质组成以及大小与人类大脑更为相似，而非啮齿动物大脑；然而，关于源自该模型的神经祖细胞的信息却很匮乏。

结果

在此，我们从一只转基因仔猪的脑外植体中分离出了GFAP阳性猪神经干细胞（NSCs），其在GFAP启动子（pGFAP-CreER）的控制下表达CreER。分离出的pGFAP-CreER神经干细胞表现出自我更新能力，并表达代表性的神经干细胞标志物，如巢蛋白（Nestin）和Sox2。药理学抑制研究表明，Notch1信号传导对于维持神经干细胞特性是必要的，而血清处理会诱导细胞分化为反应性星形胶质细胞和神经元。