Department of Evolution and Ecology, University of California, Davis, CA
Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY.
G3 (Bethesda). 2019 Jan 9;9(1):73-80. doi: 10.1534/g3.118.200818.
While significant effort has been devoted to investigating the potential influence of spatially varying selection on genomic variation, relatively little effort has been devoted to experimental analysis of putative variants or genes experiencing such selection. Previous population genetic work identified an amino acid polymorphism in the gene as one of the most strongly latitudinally differentiated SNPs in the genome of in the United States and Australia. Here we report the results of our transgenic analysis of this amino acid polymorphism. Genotypes carrying alternative alleles differed in multiple phenotypes in a direction generally consistent with phenotypic differences previously observed along latitudinal clines. These results support inferences from earlier population genomic work that this variant influences fitness, and support the idea that the alleles exhibiting clines may be likely to have pleiotropic effects that are correlated along the axes favored by natural selection.
尽管人们已经投入了大量的精力来研究空间变化选择对基因组变异的潜在影响，但在对可能经历这种选择的变异体或基因进行实验分析方面，投入的精力相对较少。之前的种群遗传学研究发现，在美国和澳大利亚的 基因组中， 基因的一个氨基酸多态性是最强烈的纬度分化 SNP 之一。在这里，我们报告了对该氨基酸多态性的转基因分析结果。携带替代 等位基因的基因型在多个表型上存在差异，方向通常与先前在纬度渐变群中观察到的表型差异一致。这些结果支持了早期群体基因组学工作的推断，即该变体影响适应性，并且支持了这样一种观点，即表现出渐变的等位基因可能具有与自然选择所青睐的轴相关的多效性效应。
