Dombrádi V, Friedrich P, Bot G
Int J Biochem. 1987;19(7):657-9. doi: 10.1016/0020-711x(87)90234-5.
The dephosphorylation of Drosophila phosphorylase a with the catalytic subunit of fruit-fly protein phosphatase-1 was inhibited by AMP, IMP, ADP, ATP, glucose-6-P, glucose-1-P and UDPG. Glucose, caffeine and glycogen did not influence the reaction. The inhibitory effect of AMP was reduced by glucose and caffeine. The above ligands acted through the modification of phosphorylase a conformation. This conclusion was drawn from the ligands' effect on the dephosphorylation of phosphohistone by Drosophila phosphatase-1 and on the tryptic digestion of fruit-fly phosphorylase a.
果蝇磷酸化酶a与果蝇蛋白磷酸酶-1催化亚基的去磷酸化反应受到AMP、IMP、ADP、ATP、6-磷酸葡萄糖、1-磷酸葡萄糖和UDPG的抑制。葡萄糖、咖啡因和糖原不影响该反应。葡萄糖和咖啡因可降低AMP的抑制作用。上述配体通过改变磷酸化酶a的构象发挥作用。这一结论是基于这些配体对果蝇磷酸酶-1催化的磷酸组蛋白去磷酸化反应以及对果蝇磷酸化酶a胰蛋白酶消化的影响得出的。
