Del Mistro A, Braunstein J D, Halwer M, Koss L G
Hum Pathol. 1987 Sep;18(9):936-40. doi: 10.1016/s0046-8177(87)80272-1.
An in situ hybridization technique was applied under stringent conditions to paraffin sections of urethral condylomata from male patients to determine the presence of DNA sequences of human papillomavirus (HPV) types 6, 11, 16, and 18. The material consisted of 15 classical condylomata acuminata, two flat condylomata, and five recurrent lesions. HPV DNA sequences could be identified in all 15 condylomata acuminata; in 13 lesions, two types of viral DNA were observed (types 6 and 11 in 12, types 6 and 18 in one). In the remaining two condylomata acuminata, only HPV type 11 was present. One of the two flat condylomata was negative with all the probes, and one was borderline-positive for HPV 6. Four of five recurrent lesions contained the same types of viral DNA as the primary lesions, albeit with slight differences in the intensity of viral expression. One lesion was negative with all probes. We conclude that urethral condylomata in males contain the same types of HPV as seen in other anogenital lesions of both sexes and that infection with two viral types is common. In situ hybridization with HPV DNA probes is applicable to archival material and therefore may prove to be of value in future epidemiologic studies comparing lesions in sexual partners. The determination of viral type may have therapeutic implications.
采用原位杂交技术，在严格条件下对男性患者尿道尖锐湿疣石蜡切片进行检测，以确定人乳头瘤病毒（HPV）6、11、16和18型的DNA序列是否存在。材料包括15例典型尖锐湿疣、2例扁平湿疣和5例复发病变。在所有15例尖锐湿疣中均能鉴定出HPV DNA序列；在13个病变中，观察到两种病毒DNA类型（12例为6型和11型，1例为6型和18型）。在其余2例尖锐湿疣中，仅存在HPV 11型。2例扁平湿疣中，1例对所有探针均呈阴性，1例对HPV 6呈临界阳性。5例复发病变中有4例含有与原发病变相同类型的病毒DNA，尽管病毒表达强度略有差异。1个病变对所有探针均呈阴性。我们得出结论，男性尿道尖锐湿疣所含HPV类型与其他男女生殖器肛门病变所见相同，且感染两种病毒类型很常见。用HPV DNA探针进行原位杂交适用于存档材料，因此可能在未来比较性伴侣病变的流行病学研究中证明有价值。病毒类型的确定可能具有治疗意义。
