Williams N, Jackson H, Rabellino E M
J Cell Physiol. 1977 Dec;93(3):435-9. doi: 10.1002/jcp.1040930315.
Modified conditions are reported for successful continuous bone marrow cultures with stem cell self-renewal and granulocyte-macrophage differentiation. Cells cultured over several weeks were found to be identical to freshly isolated bone marrow cells. Polymorphic neutrophils derived from cultures and primary bone marrow neutrophils both showed C3 AND IgG receptors and both actively phagocytosed foreign particles. Cultured and normal CFU-c were identical, both in their dose responsiveness to CFS and in their sedimentation rate characteristics.
据报道，已对成功进行连续骨髓培养的条件进行了改良，实现了干细胞自我更新和粒细胞-巨噬细胞分化。培养数周的细胞被发现与新鲜分离的骨髓细胞相同。源自培养物的多形核中性粒细胞和原代骨髓中性粒细胞均显示出C3和IgG受体，并且都能积极吞噬外来颗粒。培养的集落形成单位-细胞（CFU-c）和正常CFU-c在对集落刺激因子（CFS）的剂量反应性及其沉降速率特征方面均相同。
