Department of Botany, Panjab University, Chandigarh, 160014, India.
Department of Environment Studies, Panjab University, Chandigarh, 160014, India.
Environ Sci Pollut Res Int. 2019 Jan;26(1):456-463. doi: 10.1007/s11356-018-3562-1. Epub 2018 Nov 7.
We examined the possible role of monoterpene β-pinene in providing protection against Cr(VI) toxicity in maize (Zea mays). Treatment with β-pinene (10 μM) significantly alleviated Cr(VI) accumulation and recuperated Cr(VI) caused decline in root and coleoptile growth in maize. β-Pinene addition caused a decline in Cr(VI)-induced accumulation of superoxide anion, hydroxyl ion, hydrogen peroxide and confirmed by in-situ detection of ROS using histochemical localization. It suggested that the β-pinene quenches/neutralizes enhanced ROS generated under Cr(VI) exposure. β-Pinene also reduced Cr(VI)-induced electrolyte leakage, thereby suggesting its role in membrane stabilization. Further, β-pinene regulated the activity of scavenging enzymes, thereby suggesting a role in modulating Cr(VI)-induced oxidative damage. In conclusion, our results suggest that the addition of β-pinene has a protective role against Cr(VI) stress and provides resistance to maize against Cr(VI) toxicity.
我们研究了单萜 β-蒎烯在提供玉米（Zea mays）对 Cr(VI)毒性的保护作用方面的可能作用。用β-蒎烯（10 μM）处理可显著减轻 Cr(VI)的积累，并恢复 Cr(VI)引起的玉米根和胚芽鞘生长的下降。β-蒎烯的添加导致 Cr(VI)诱导的超氧阴离子、羟基离子、过氧化氢的积累减少，并通过使用 ROS 的原位检测进行了证实。这表明β-蒎烯淬灭/中和了 Cr(VI)暴露下增强的 ROS 的产生。β-蒎烯还减少了 Cr(VI)诱导的电解质泄漏，从而表明其在膜稳定化方面的作用。此外，β-蒎烯调节了清除酶的活性，从而表明其在调节 Cr(VI)诱导的氧化损伤方面的作用。总之，我们的结果表明，添加β-蒎烯对 Cr(VI)胁迫具有保护作用，并为玉米提供了对 Cr(VI)毒性的抗性。
