棕色固氮菌和肺炎克雷伯氏菌中固氮特异性基因的比较组织：nifUSV基因的DNA序列
Comparative organization of nitrogen fixation-specific genes from Azotobacter vinelandii and Klebsiella pneumoniae: DNA sequence of the nifUSV genes.
作者信息
Beynon J, Ally A, Cannon M, Cannon F, Jacobson M, Cash V, Dean D
出版信息
J Bacteriol. 1987 Sep;169(9):4024-9. doi: 10.1128/jb.169.9.4024-4029.1987.
Abstract
In the facultative anaerobe Klebsiella pneumoniae 17 nitrogen fixation-specific genes (nif genes) have been identified. Homologs to 12 of these genes have now been isolated from the aerobic diazotroph Azotobacter vinelandii. Comparative studies have indicated that these diverse microorganisms share striking similarities in the genetic organization of their nif genes and in the primary structure of their individual nif gene products. In this study the complete nucleotide sequence of the nifUSV gene clusters from both K. pneumoniae and A. vinelandii were determined. These genes are identically organized on their respective genomes, and the individual genes and their products exhibit a high degree of interspecies sequence homology.
摘要
在兼性厌氧菌肺炎克雷伯菌中，已鉴定出17个固氮特异性基因（nif基因）。目前已从好氧固氮菌维氏固氮菌中分离出其中12个基因的同源物。比较研究表明，这些不同的微生物在其nif基因的遗传组织及其单个nif基因产物的一级结构上具有惊人的相似性。在本研究中，测定了肺炎克雷伯菌和维氏固氮菌nifUSV基因簇的完整核苷酸序列。这些基因在各自的基因组上组织方式相同，并且各个基因及其产物表现出高度的种间序列同源性。
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