A Ratiometric Fluorescent Probe for Cisplatin: Investigating the Intracellular Reduction of Platinum(IV) Prodrug Complexes.

The Pt prodrug strategy has emerged as an excellent alternative to tackle the problems associated with conventional Pt drug therapy. However, there is a lack of tools to study how this new class of Pt drugs are processed at the cellular level. Herein, we report the first ratiometric probe for cisplatin detection and use it to investigate Pt anticancer complexes in biological systems. The probe was able to distinguish between cisplatin and its Pt derivatives, allowing us to probe the intracellular reduction of Pt prodrug complexes. The correlation between the amount of active Pt species available after intracellular reduction of Pt complexes and their cytotoxicity and the role glutathione plays in the reduction of Pt complexes were investigated.