Luo Yi-Pey
Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan (ROC).
J Vector Ecol. 2018 Dec;43(2):328-333. doi: 10.1111/jvec.12317.
Successful colonies of the biting midge Forcipomyia taiwana (Shiraki) were established and maintained in the laboratory by feeding blood with an artificial blood-feeding apparatus, rearing larvae on a soil substrate employing algae liquid, and setting suitable mating cages. The feeding rates of F. taiwana fed on pig blood (69.9%) and artificial blood (72.7%) were not significantly different from those fed on human blood (67.0%). The mean numbers of adults produced by females fed on the artificial blood and the human blood were 32.0 and 33.0, respectively. The algae liquid, Chlorella vulgaris, was suitable for rearing larvae, with larval hatching rate, pupation rate, and emergence rate of midges fed with artificial blood and human blood meal cohorts observed as 76.0%-88.8%, 98.2%-96.4%, and 98.0-94.3%, respectively. Swarming and copulation occurred 1 h before and 2 h after the lights were turned on (07:00-10:00). The average female mating rates were approximately 50-60%, and males were observed to mate with multiple females.
通过使用人工采血装置喂食血液、在含有藻类液体的土壤基质上饲养幼虫以及设置合适的交配笼，成功在实验室建立并维持了台湾蠛蠓（白氏）的种群。以猪血（69.9%）和人造血（72.7%）为食的台湾蠛蠓的取食率与以人血（67.0%）为食的取食率没有显著差异。以人造血和人血为食的雌虫产生的成虫平均数量分别为32.0和33.0。小球藻藻类液体适合饲养幼虫，观察到以人造血和人血为食的幼虫的孵化率、化蛹率和羽化率分别为76.0%-88.8%、98.2%-96.4%和98.0%-94.3%。在开灯前1小时和开灯后2小时（07:00-10:00）出现群聚和交配现象。雌虫的平均交配率约为50%-60%，观察到雄虫与多只雌虫交配。
