Wang Szu-Chieh, Ching Yung-Hao, Krishnaraj Preethi, Chen Guan-Yu, Radhakrishnan Anna Shiny, Lee Hsien-Min, Tu Wu-Chun, Lin Ming-Der
Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 97004, Taiwan.
Department of Medical Research, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan.
Insects. 2020 Feb 5;11(2):106. doi: 10.3390/insects11020106.
is an irritating hematophagous midge that preferentially attacks humans and affects leisure industries in Taiwan. Understanding the female reproductive biology of such pests would facilitate the development of pest control strategies. However, knowledge about oogenesis in the genus is unavailable. Accordingly, we examined the ovariole structure and features of oogenesis in terms of the oocyte and the nurse cell. After being blood-fed, we observed a high degree of gonotrophic harmony-the synchronization of developing follicles. The follicle of the has only one nurse cell connected to the oocyte, which is distinct among hematophagous midges. In the nurse cell, we identified the perinuclear localization of the germline marker, Vasa. The Vasa localization is reminiscent of the nuclear envelope-associated nuage observed by electron microscopy. To determine whether Vasa (FtVasa) is an authentic nuage component, we produced transgenic flies expressing FtVasa in the female germline and proved that FtVasa was able to be localized to nuage. By characterizing the oogenesis and Vasa expression in the germline cells of , this study extends knowledge about the female reproductive biology of hematophagous midges.
是一种令人讨厌的吸血蠓，它优先攻击人类并影响台湾的休闲产业。了解此类害虫的雌性生殖生物学将有助于制定害虫控制策略。然而，关于该属卵子发生的知识尚不可得。因此，我们从卵母细胞和滋养细胞方面研究了卵巢管结构和卵子发生特征。在吸食血液后，我们观察到高度的生殖营养协调——发育卵泡的同步化。该蠓的卵泡只有一个与卵母细胞相连的滋养细胞，这在吸血蠓中是独特的。在滋养细胞中，我们鉴定出生殖系标记物Vasa的核周定位。Vasa的定位让人联想到通过电子显微镜观察到的与核膜相关的生殖质。为了确定该蠓的Vasa（FtVasa）是否是真正的生殖质成分，我们构建了在雌性生殖系中表达FtVasa的转基因果蝇，并证明FtVasa能够定位于该蠓的生殖质。通过表征该蠓生殖系细胞中的卵子发生和Vasa表达，本研究扩展了关于吸血蠓雌性生殖生物学的知识。
