Nie Lan-Bi, Zou Yang, Hou Jun-Ling, Liang Qin-Li, Cong Wei, Zhu Xing-Quan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, PR China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, 130118, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, PR China.
Acta Trop. 2019 Feb;190:80-82. doi: 10.1016/j.actatropica.2018.11.002. Epub 2018 Nov 5.
Neospora caninum is an intracellular protozoan parasite with a worldwide distribution, which can cause abortion and stillbirth in ruminants. However, little is known of N. caninum infection in raccoon dogs in China. Thus, a total of 1181 serum samples of farmed raccoon dogs were collected from the major production areas of Jilin, Changchun in Jilin province, and Rizhao, Weihai and Yantai in Shandong province in China. The antibodies to N. caninum were examined by a competitive-inhibition enzyme-linked immunoassay (cELISA). While the N. caninum seroprevalence in farmed raccoon dogs from different regions (cities) were not statistically significant, farmed raccoon dogs in Shandong province had a significantly higher N. caninum seroprevalence (8.99%, 95% CI 7.13-10.85) than those in Jilin province (4.46%, 95% CI 1.99-6.93) (P < 0.05). N. caninum seroprevalence in male and female raccoon dogs were 7.91% (95% CI 5.99-9.83) and 8.06% (95% CI 5.46-10.66), respectively. In addition, the seroprevalence of N. caninum infection in infancy, youth and adult raccoon dogs was 7.32% (95% CI 4.31-10.33), 7.93% (95% CI 5.44-10.42) and 8.41% (95% CI 5.82-11.00), respectively. This is the first report of N. caninum seroprevalence in farmed raccoon dogs in China, which provided baseline data for the prevention of N. caninum infection in raccoon dogs in China.
犬新孢子虫是一种细胞内原生动物寄生虫，在全球范围内均有分布，可导致反刍动物流产和死产。然而，在中国，关于貉感染犬新孢子虫的情况却知之甚少。因此，我们从中国吉林省主要产区、吉林省长春市以及山东省日照市、威海市和烟台市共采集了1181份养殖貉的血清样本。采用竞争抑制酶联免疫吸附试验（cELISA）检测犬新孢子虫抗体。虽然来自不同地区（城市）的养殖貉中犬新孢子虫血清阳性率无统计学差异，但山东省养殖貉的犬新孢子虫血清阳性率（8.99%，95%可信区间7.13 - 10.85）显著高于吉林省（4.46%，95%可信区间1.99 - 6.93）（P < 0.05）。雄性和雌性貉的犬新孢子虫血清阳性率分别为7.91%（95%可信区间5.99 - 9.83）和8.06%（95%可信区间5.46 - 10.66）。此外，幼年、青年和成年貉的犬新孢子虫感染血清阳性率分别为7.32%（95%可信区间4.31 - 10.33）、7.93%（95%可信区间5.44 - 10.42）和8.41%（95%可信区间5.82 - 11.00）。这是中国养殖貉中犬新孢子虫血清阳性率的首次报道，为中国预防貉感染犬新孢子虫提供了基线数据。
