Institut für Molekulare Zellbiologie, CMB-Center for Molecular Biomedicine, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, Hans-Knöll Straße 2, D-07745 Jena, Germany.
Institut für Molekulare Zellbiologie, CMB-Center for Molecular Biomedicine, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, Hans-Knöll Straße 2, D-07745 Jena, Germany.
Curr Opin Cell Biol. 2019 Apr;57:16-24. doi: 10.1016/j.ceb.2018.10.005. Epub 2018 Nov 5.
Advances in resolving crystal structures of GPCRs and their binding partners as well as improvements in live-cell microscopy and the fluorescent proteins pallet has greatly driven new ideas for designing optical sensors for the same. Sensors have been developed to monitor ligand binding as well as the ensuing ligand-induced conformational changes in GPCRs, G-proteins and arrestins. In this review we will highlight the functionality of such sensor designs starting from monitoring ligand binding to receptor activation and interaction with arrestins. Furthermore, we will highlight the importance of sensor designs to monitor receptor-dependent arrestin conformations and give an idea about the various detected arrestin conformations and their possible implications.
解析 GPCR 及其配体晶体结构方面的进展，以及活细胞显微镜和荧光蛋白库的改进，极大地推动了设计用于检测 GPCR 的光学传感器的新思路。已经开发出了用于监测配体结合以及随后配体诱导 GPCR、G 蛋白和阻滞蛋白构象变化的传感器。在这篇综述中，我们将从监测配体结合到受体激活以及与阻滞蛋白相互作用开始，突出此类传感器设计的功能。此外，我们还将突出传感器设计在监测受体依赖性阻滞蛋白构象方面的重要性，并介绍各种检测到的阻滞蛋白构象及其可能的影响。
