Key Laboratory of Reproductive Genetics (Ministry of Education), Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Institute of Environmental Health, Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.
Environ Pollut. 2018 Dec;243(Pt B):1878-1886. doi: 10.1016/j.envpol.2018.09.109. Epub 2018 Oct 3.
Polycyclic aromatic hydrocarbons (PAHs) mediated ovarian toxicity has been demonstrated in animal experiments. However, this issue has not been assessed in humans. Based on the National Health and Nutrition Examination Survey (NHANES) 2003-2012, data analysis was restricted to 1221 general U.S. women aged 35-65 years with complete data of interest. Levels of nine PAH metabolites in spot urine specimens were measured by isotope dilution gas chromatography/tandem mass spectrometry (GC-MS/MS). Self-reported information on the menopause status and age at menopause were obtained during interview. Cox proportional hazards regression was employed to assess the associations between PAH levels and natural menopause. Compared with women in the first quartile, subjects in the highest quartile of 1-Hydroxynapthalene [hazard ratio (HR) = 1.46, 95% confidence interval (CI) = 1.06 to 2.01], 2-Hydroxynapthalene (HR = 1.51, 95% CI = 1.12 to 2.05) and 3-Hydroxyfluorene (HR = 1.51, 95% CI = 1.06 to 2.16), or in the second quartile of 9-Hydroxyfluorene (HR = 1.53, 95% CI = 1.05 to 2.22), had elevated risks of earlier onset of natural menopause. Our findings suggested positive associations between urinary PAH levels and earlier age at natural menopause in the general U.S. women. Prospective studies are warranted to confirm the causality in the future.
多环芳烃 (PAHs) 介导的卵巢毒性已在动物实验中得到证实。然而，这一问题在人类中尚未得到评估。基于 2003-2012 年的全国健康和营养调查 (NHANES)，数据分析仅限于 1221 名年龄在 35-65 岁之间、有完整相关数据的美国普通女性。采用同位素稀释气相色谱/串联质谱法 (GC-MS/MS) 测量尿液标本中 9 种 PAH 代谢物的水平。在访谈期间获得了关于绝经状态和绝经年龄的自我报告信息。采用 Cox 比例风险回归评估 PAH 水平与自然绝经之间的关联。与处于第一四分位的女性相比，处于 1-羟基萘（HR=1.46，95%置信区间[CI]=1.06 至 2.01）、2-羟基萘（HR=1.51，95%CI=1.12 至 2.05）和 3-羟基芴（HR=1.51，95%CI=1.06 至 2.16）最高四分位的受试者或 9-羟基芴（HR=1.53，95%CI=1.05 至 2.22）第二四分位的受试者，其自然绝经年龄较早的风险升高。我们的研究结果表明，美国普通女性尿液中 PAH 水平与自然绝经年龄较早之间存在正相关。未来需要进行前瞻性研究以确认因果关系。
