诺尔斯菌素（链丝菌素）对敏感和耐药大肠杆菌菌株外膜的影响。

Effect of nourseothricin (streptothricin) on the outer membrane of sensitive and resistant Escherichia coli strains.

作者信息

Seltmann G, Wolter E J

出版信息

J Basic Microbiol. 1987;27(3):139-46. doi: 10.1002/jobm.3620270305.

DOI:10.1002/jobm.3620270305

Abstract

Nourseothricin (streptothricin) causes disturbances (perforations) in the outer membrane of sensitive E. coli strains allowing lysozyme and deoxycholate, but not the periplasmic alkaline phosphatase to penetrate. EDTA slightly increases, but Mg++ ions slightly decrease this effect. The cell walls of three from four nourseothricin-resistant strains do not become permeable under these conditions, but remain sensitive against TRIS/EDTA. Nourseothricin is supposed to pass the outer membrane of sensitive bacteria via some kind of "self-promoting" pathway. This way can (but need not) be blocked in resistant strains.

摘要

诺尔斯菌素（链丝菌素）会导致敏感大肠杆菌菌株外膜出现扰动（穿孔），使溶菌酶和脱氧胆酸盐能够穿透，但周质碱性磷酸酶不能穿透。乙二胺四乙酸（EDTA）会略微增强这种作用，而镁离子（Mg++）则会略微减弱这种作用。在这些条件下，四株耐诺尔斯菌素菌株中有三株的细胞壁不会变得通透，但对三羟甲基氨基甲烷/乙二胺四乙酸（TRIS/EDTA）仍敏感。诺尔斯菌素被认为是通过某种“自我促进”途径穿过敏感细菌的外膜。在耐药菌株中，这种途径可能（但不一定）会被阻断。