Tapia-Morales S, López-Landavery E A, Giffard-Mena I, Ramírez-Álvarez N, Gómez-Reyes R J E, Díaz F, Galindo-Sánchez C E
Centro de Investigación Científica y Educación Superior de Ensenada, Laboratorio de Genómica Marina, Carretera Ensenada-Tijuana, C.P. 22860 Ensenada, B. C., Mexico; Universidad Autónoma de Baja California, Facultad de Ciencias Marinas, Laboratorio de Patología Experimental Acuícola, Carretera Ensenada-Tijuana No. 3917, C.P. 22860 Ensenada, B. C., Mexico.
Centro de Investigación Científica y Educación Superior de Ensenada, Laboratorio de Genómica Marina, Carretera Ensenada-Tijuana, C.P. 22860 Ensenada, B. C., Mexico.
Mar Genomics. 2019 Feb;43:9-18. doi: 10.1016/j.margen.2018.10.004. Epub 2018 Nov 5.
The Crassostrea virginica oyster has biological and economic importance in the Gulf of Mexico, an area with a high extraction and production of hydrocarbons. Exposure to hydrocarbons affects the reproductive processes in bivalves. In C. virginica, the effect of hydrocarbons on the gonad of the undifferentiated organism has not been evaluated to determine the possible damage during the maturation process. To evaluate this effect, RNA-seq data was generated from C. virginica gonads exposed to a 200 μg/L of hydrocarbons at different exposure times (7, 14 and 21 days) and a control treatment (without hydrocarbons). The analysis of the gonad transcriptome showed the negative effect of hydrocarbons on maturation, with a sub-expression of 22 genes involved in different stages of this process. Additionally, genes in the immune system were down-regulated, which may indicate that exposure to hydrocarbons causes immunosuppression in bivalves. A group of oxidative stress genes was also reduced. These data contribute to a better understanding of the effect of hydrocarbons on the reproductive process in bivalves and, at the same time, allow us to identify possible biomarkers associated with hydrocarbon contamination in the gonad of C. virginica.
弗吉尼亚海湾牡蛎在墨西哥湾具有生物学和经济重要性，该区域碳氢化合物的开采和产量很高。接触碳氢化合物会影响双壳贝类的繁殖过程。在弗吉尼亚海湾牡蛎中，尚未评估碳氢化合物对未分化生物体性腺的影响，以确定成熟过程中可能的损害。为了评估这种影响，从暴露于200μg/L碳氢化合物不同时间（7天、14天和21天）的弗吉尼亚海湾牡蛎性腺以及对照处理（无碳氢化合物）中生成了RNA测序数据。性腺转录组分析显示碳氢化合物对成熟有负面影响，参与该过程不同阶段的22个基因表达下调。此外，免疫系统中的基因也被下调，这可能表明接触碳氢化合物会导致双壳贝类免疫抑制。一组免疫相关基因也减少了。这些数据有助于更好地理解碳氢化合物对双壳贝类繁殖过程的影响，同时使我们能够识别与弗吉尼亚海湾牡蛎性腺中碳氢化合物污染相关的可能生物标志物。
