Mazella J, Amar S, Bozou J C, Kitabgi P, Vincent J P
J Recept Res. 1987;7(1-4):157-65. doi: 10.3109/10799898709054984.
Membranes prepared from mammalian brain or intestine contain two types of specific binding sites for neurotensin that differ by their affinity and by their sensitivity to sodium ions, GTP, and the antihistamine drug levocabastine. Only the high affinity sites are present in cell cultures and in soluble extracts of CHAPS-treated membranes. These sites represent functional neurotensin receptors coupled to GTP-binding proteins that regulate intracellular levels of cAMP, cGMP and inositol phosphates in neuroblastoma N1E115 cells. The molecular weight of neurotensin receptors in cells and membrane preparations of various origin is about 110,000.
从哺乳动物大脑或肠道制备的膜含有两种类型的神经降压素特异性结合位点，它们在亲和力以及对钠离子、鸟苷三磷酸（GTP）和抗组胺药物左卡巴斯汀的敏感性方面存在差异。只有高亲和力位点存在于细胞培养物和经3-[(3-胆酰胺丙基)二甲氨基]-1-丙磺酸（CHAPS）处理的膜的可溶性提取物中。这些位点代表与GTP结合蛋白偶联的功能性神经降压素受体，该受体调节神经母细胞瘤N1E115细胞中细胞内环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）和肌醇磷酸的水平。各种来源的细胞和膜制剂中的神经降压素受体的分子量约为110,000。
