Departamento de Bioquímica e Biologia molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil.
Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil.
Clin Chim Acta. 2019 Jan;488:90-97. doi: 10.1016/j.cca.2018.10.032. Epub 2018 Oct 25.
Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease, where there is irreversible breakdown of immunological self-tolerance. Extracellular adenosine triphosphate (ATP) and adenosine are signaling molecules that play an important part in the immune response. During inflammation and the immune response, a group of enzymes control these molecules, including ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), E-5'-nucleotidase, and ecto-adenosine deaminase (E-ADA). We determined the activity and expression of E-NTPDase, the expression of E-5'-nucleotidase, the activity of E-ADA in lymphocytes and serum of SLE patients.
This study involved 35 patients with SLE and 30 healthy subjects as a control group. E-NTPDase activity and expression were increased in lymphocytes from SLE patients (31% and 37% for activity and expression, respectively) compared with the control group.
An approximately 42% increase in E-ADA activity in lymphocytes was observed in SLE patients compared with the control group, in serum the ADA activity was decreased by 57% in SLE patients. Expression of E-5'-nucleotidase was not changed in SLE patients.
E-NTPDase and E-ADA perform key functions in the modulation of the immune and inflammatory response in SLE.
系统性红斑狼疮（SLE）是一种炎症性自身免疫性疾病，其中免疫自身耐受发生不可逆转的破坏。细胞外三磷酸腺苷（ATP）和腺苷是信号分子，在免疫反应中发挥重要作用。在炎症和免疫反应期间，一组酶控制这些分子，包括外核苷酸三磷酸二磷酸水解酶（E-NTPDase）、E-5′-核苷酸酶和外腺苷脱氨酶（E-ADA）。我们测定了 SLE 患者淋巴细胞和血清中 E-NTPDase 的活性和表达、E-5′-核苷酸酶的表达以及 E-ADA 的活性。
本研究纳入 35 例 SLE 患者和 30 例健康受试者作为对照组。与对照组相比，SLE 患者淋巴细胞中的 E-NTPDase 活性（增加 31%）和表达（增加 37%）增加。
与对照组相比，SLE 患者淋巴细胞中的 E-ADA 活性增加约 42%，而 SLE 患者血清中的 ADA 活性降低 57%。SLE 患者的 E-5′-核苷酸酶表达没有改变。
E-NTPDase 和 E-ADA 在 SLE 中调节免疫和炎症反应中发挥关键作用。
