André C, Marullo S, Guillet J G, Convents A, Lauwereys M, Kaveri S, Hoebeke J, Strosberg A D
J Recept Res. 1987;7(1-4):89-103. doi: 10.3109/10799898709054981.
Muscarinic receptors have been purified from calf forebrain plasma cell membranes by affinity chromatography on a dexetimide-agarose gel. SDS-PAGE analysis showed a single 70 kDa band. Monoclonal antibodies have been prepared against these affinity purified 70 kDa protein(s). One antibody, M-35, immunoprecipitated up to 80% of digitonin-solubilized muscarinic receptors. M-35 had agonist-like effects on guinea-pig myometrium: it increased the intracellular cyclic GMP content, decreased prostaglandin-induced cyclic AMP accumulation and caused muscle contractions. The two first effects were inhibited by atropine. M-35 was used to visualize muscarinic receptors at the surface of human fibroblastic cells. In the particular cell line used, the receptors have a low affinity for pirenzepine, were negatively coupled to adenylate cyclase and mediated increase in the phosphatidyl-inositol breakdown.
通过在右旋苯乙托品 - 琼脂糖凝胶上进行亲和层析，已从小牛前脑浆细胞膜中纯化出毒蕈碱受体。SDS - PAGE分析显示出一条单一的70 kDa条带。已制备出针对这些亲和纯化的70 kDa蛋白的单克隆抗体。一种抗体M - 35可免疫沉淀高达80%的洋地黄皂苷增溶的毒蕈碱受体。M - 35对豚鼠子宫肌层有类似激动剂的作用：它增加细胞内环鸟苷酸含量，降低前列腺素诱导的环腺苷酸积累并引起肌肉收缩。前两种作用可被阿托品抑制。M - 35用于观察人成纤维细胞表面的毒蕈碱受体。在所使用的特定细胞系中，这些受体对哌仑西平具有低亲和力，与腺苷酸环化酶负偶联，并介导磷脂酰肌醇分解增加。
